Nitrogen removal characteristics and biofilm analysis of a membrane-aerated biofilm reactor applicable to high-strength nitrogenous wastewater treatment

Akihiko Terada, Kazuaki Hibiya, Jun Nagai, Satoshi Tsuneda, Akira Hirata

    Research output: Contribution to journalArticle

    148 Citations (Scopus)

    Abstract

    A membrane-aerated biofilm reactor (MABR) capable of simultaneous nitrification and denitrification in a single reactor vessel was developed to investigate the characteristics of nitrogen removal from high-strength nitrogenous wastewater, and biofilm analysis using microelectrodes and the fluorescence in situ hybridization (FISH) technique was performed. Mean removal percentages of total organic carbon (TOC) and nitrogen were 96% and 83% at removal rates of 5.76 g-C m-2 d-1 and 4.48 g-N m-2 d-1, respectively. For stable removal efficiency, constant washing of the biofilm was needed. Dissolved oxygen microelectrode measurement revealed that the biofilm thickness was about 1600 μm, and that oxygen penetrated about 300 to 700 μm from the outer surface of the membrane. Furthermore, FISH analysis revealed that ammonia-oxidizing bacteria (AOB) were located near the outer surface of the membrane, whereas other bacteria were located from the inner to the outer part of the biofilm. Combining these results demonstrated that simultaneous nitrification and denitrification occurred in the biofilm of the MABR system. In addition, stoichiometric analysis revealed that after 130 d the free ammonia (FA) concentration ranged within the concentration causing inhibition of the growth of nitrite oxidizing bacteria (NOB) and that AOB consumed 86% of the oxygen supplied through the intra-membrane. These results indicate that nitrogen removal not via nitrate but via nitrite was mainly achieved in the MABR system.

    Original languageEnglish
    Pages (from-to)170-178
    Number of pages9
    JournalJournal of Bioscience and Bioengineering
    Volume95
    Issue number2
    DOIs
    Publication statusPublished - 2003

    Fingerprint

    Nitrogen removal
    Biofilms
    Waste Water
    Wastewater treatment
    Nitrogen
    Membranes
    Bacteria
    Ammonia
    Nitrification
    Denitrification
    Microelectrodes
    Oxygen
    Nitrites
    Fluorescence In Situ Hybridization
    Fluorescence
    Dissolved oxygen
    Organic carbon
    Washing
    Nitrates
    Wastewater

    Keywords

    • Fluorescence in situ hybridization (FISH)
    • High-strength nitrogenous wastewater
    • Membrane-aerated biofilm reactor (MABR)
    • Microelectrode
    • Nitrogen removal

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering

    Cite this

    Nitrogen removal characteristics and biofilm analysis of a membrane-aerated biofilm reactor applicable to high-strength nitrogenous wastewater treatment. / Terada, Akihiko; Hibiya, Kazuaki; Nagai, Jun; Tsuneda, Satoshi; Hirata, Akira.

    In: Journal of Bioscience and Bioengineering, Vol. 95, No. 2, 2003, p. 170-178.

    Research output: Contribution to journalArticle

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    abstract = "A membrane-aerated biofilm reactor (MABR) capable of simultaneous nitrification and denitrification in a single reactor vessel was developed to investigate the characteristics of nitrogen removal from high-strength nitrogenous wastewater, and biofilm analysis using microelectrodes and the fluorescence in situ hybridization (FISH) technique was performed. Mean removal percentages of total organic carbon (TOC) and nitrogen were 96{\%} and 83{\%} at removal rates of 5.76 g-C m-2 d-1 and 4.48 g-N m-2 d-1, respectively. For stable removal efficiency, constant washing of the biofilm was needed. Dissolved oxygen microelectrode measurement revealed that the biofilm thickness was about 1600 μm, and that oxygen penetrated about 300 to 700 μm from the outer surface of the membrane. Furthermore, FISH analysis revealed that ammonia-oxidizing bacteria (AOB) were located near the outer surface of the membrane, whereas other bacteria were located from the inner to the outer part of the biofilm. Combining these results demonstrated that simultaneous nitrification and denitrification occurred in the biofilm of the MABR system. In addition, stoichiometric analysis revealed that after 130 d the free ammonia (FA) concentration ranged within the concentration causing inhibition of the growth of nitrite oxidizing bacteria (NOB) and that AOB consumed 86{\%} of the oxygen supplied through the intra-membrane. These results indicate that nitrogen removal not via nitrate but via nitrite was mainly achieved in the MABR system.",
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