NO and CO binding profiles of hemoglobin vesicles as artificial oxygen carriers

Hiromi Sakai, Atsushi Sato, Peter Sobolewski, Shinji Takeoka, John A. Frangos, Koichi Kobayashi, Marcos Intaglietta, Eishun Tsuchida

    Research output: Contribution to journalArticle

    19 Citations (Scopus)

    Abstract

    Hemoglobin vesicles (HbVs) are artificial oxygen carriers encapsulating purified and concentrated Hb solution in phospholipid vesicles (liposomes). We examined in-vitro reaction profiles of a formulation of HbV with NO and CO in anaerobic and aerobic conditions using stopped-flow spectrophotometry and a NO electrode. Reaction rate constants of NO to deoxygenated and oxygenated HbV were considerably smaller than those of cell-free Hb because of the intracellular NO-diffusion barrier. The reaction of CO with deoxygenated HbV was slightly slower than that of cell-free Hb solely because of the co-encapsulated allosteric effector, pyridoxal 5'-phosphate. The NO depletion in an aerobic condition in the presence of empty vesicles was monitored using a NO electrode, showing that the hydrophobic bilayer membrane of HbV, which might have higher gas solubility, does not markedly facilitate the O2 and NO reaction, and that the intracellular Hb is the major component of NO depletion. In conclusion, HbV shows retarded gas reactions, providing some useful information to explain the absence of vasoconstriction and hypertension when they are intravenously injected.

    Original languageEnglish
    Pages (from-to)1441-1447
    Number of pages7
    JournalBiochimica et Biophysica Acta - Proteins and Proteomics
    Volume1784
    Issue number10
    DOIs
    Publication statusPublished - 2008 Oct

    Fingerprint

    Carbon Monoxide
    Electrodes
    Hemoglobins
    Gases
    Oxygen
    Blood Substitutes
    Pyridoxal Phosphate
    Diffusion barriers
    Spectrophotometry
    Vasoconstriction
    Liposomes
    Solubility
    Reaction rates
    Rate constants
    Phospholipids
    Hypertension
    Membranes
    In Vitro Techniques

    Keywords

    • Artificial red cells
    • Blood substitutes
    • CO
    • Liposome
    • NO
    • Vasoconstriction

    ASJC Scopus subject areas

    • Biochemistry
    • Biophysics
    • Genetics

    Cite this

    NO and CO binding profiles of hemoglobin vesicles as artificial oxygen carriers. / Sakai, Hiromi; Sato, Atsushi; Sobolewski, Peter; Takeoka, Shinji; Frangos, John A.; Kobayashi, Koichi; Intaglietta, Marcos; Tsuchida, Eishun.

    In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1784, No. 10, 10.2008, p. 1441-1447.

    Research output: Contribution to journalArticle

    Sakai, H, Sato, A, Sobolewski, P, Takeoka, S, Frangos, JA, Kobayashi, K, Intaglietta, M & Tsuchida, E 2008, 'NO and CO binding profiles of hemoglobin vesicles as artificial oxygen carriers', Biochimica et Biophysica Acta - Proteins and Proteomics, vol. 1784, no. 10, pp. 1441-1447. https://doi.org/10.1016/j.bbapap.2008.03.007
    Sakai, Hiromi ; Sato, Atsushi ; Sobolewski, Peter ; Takeoka, Shinji ; Frangos, John A. ; Kobayashi, Koichi ; Intaglietta, Marcos ; Tsuchida, Eishun. / NO and CO binding profiles of hemoglobin vesicles as artificial oxygen carriers. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2008 ; Vol. 1784, No. 10. pp. 1441-1447.
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