No transcription-translation feedback in circadian rhythm of KaiC phosphorylation

Jun Tomita, Masato Nakajima, Takao Kondo, Hideo Iwasaki

Research output: Contribution to journalArticle

329 Citations (Scopus)

Abstract

An autoregulatory transcription-translation feedback loop is thought to be essential in generating circadian rhythms in any model organism. In the cyanobacterium Synechococcus elongatus, the essential clock protein KaiC is proposed to form this type of transcriptional negative feedback. Nevertheless, we demonstrate here temperature-compensated, robust circadian cycling of KaiC phosphorylation even without kaiBC messenger RNA accumulation under continuous dark conditions. This rhythm persisted in the presence of a transcription or translation inhibitor. Moreover, kinetic profiles in the ratio of KaiC autophosphorylation-dephosphorylation were also temperature compensated in vitro. Thus, the cyanobacterial clock can keep time independent of de novo transcription and translation processes.

Original languageEnglish
Pages (from-to)251-254
Number of pages4
JournalScience
Volume307
Issue number5707
DOIs
Publication statusPublished - 2005 Jan 14
Externally publishedYes

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Circadian Rhythm
Phosphorylation
Synechococcus
Temperature
Cyanobacteria
Messenger RNA
Proteins
In Vitro Techniques

ASJC Scopus subject areas

  • General

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No transcription-translation feedback in circadian rhythm of KaiC phosphorylation. / Tomita, Jun; Nakajima, Masato; Kondo, Takao; Iwasaki, Hideo.

In: Science, Vol. 307, No. 5707, 14.01.2005, p. 251-254.

Research output: Contribution to journalArticle

Tomita, Jun ; Nakajima, Masato ; Kondo, Takao ; Iwasaki, Hideo. / No transcription-translation feedback in circadian rhythm of KaiC phosphorylation. In: Science. 2005 ; Vol. 307, No. 5707. pp. 251-254.
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