Non-CpG methylation occurs in the regulatory region of the Sry gene

Koichiro Nishino, Naoko Hattori, Shun Sato, Yoshikazu Arai, Satoshi Tanaka, Andras Nagy, Kunio Shiota

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The Sry (sex determining region on Y chromosome) gene is a master gene for sex determination. We previously reported that the Sry gene has tissue-dependent and differentially methylated regions (T-DMRs) by analyzing the DNA methylation states at CpG sites in the promoter regions. In this study, we found unique non-CpG methylation at the internal cytosine in the 5'-CCTGG-3' pentanucleotide sequence in the Sry T-DMR. This non-CpG methylation was detected in four mouse strains (ICR, BALB/c, DBA2 and C3H), but not in two strains (C57BL/6 and 129S1), suggesting that the CCTGG methylation is tentative and unstable. Interestingly, this CCTGG methylation was associated with demethylation of the CpG sites in the Sry T-DMR in the developmental process. A methylationmediated promoter assay showed that the CCTGG methylation promotes gene expression. Our finding shows that non-CpG methylation has unique characteristic and is still conserved in mammals.

Original languageEnglish
Pages (from-to)586-593
Number of pages8
JournalJournal of Reproduction and Development
Volume57
Issue number5
DOIs
Publication statusPublished - 2011 Jun 3
Externally publishedYes

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methylation
genes
promoter regions
gender
cytosine
Y chromosome
DNA methylation
mammals
gene expression
mice
assays
tissues

Keywords

  • DNA methylation
  • Non-CpG methylation
  • Sry

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Nishino, K., Hattori, N., Sato, S., Arai, Y., Tanaka, S., Nagy, A., & Shiota, K. (2011). Non-CpG methylation occurs in the regulatory region of the Sry gene. Journal of Reproduction and Development, 57(5), 586-593. https://doi.org/10.1262/jrd.11-033A

Non-CpG methylation occurs in the regulatory region of the Sry gene. / Nishino, Koichiro; Hattori, Naoko; Sato, Shun; Arai, Yoshikazu; Tanaka, Satoshi; Nagy, Andras; Shiota, Kunio.

In: Journal of Reproduction and Development, Vol. 57, No. 5, 03.06.2011, p. 586-593.

Research output: Contribution to journalArticle

Nishino, K, Hattori, N, Sato, S, Arai, Y, Tanaka, S, Nagy, A & Shiota, K 2011, 'Non-CpG methylation occurs in the regulatory region of the Sry gene', Journal of Reproduction and Development, vol. 57, no. 5, pp. 586-593. https://doi.org/10.1262/jrd.11-033A
Nishino, Koichiro ; Hattori, Naoko ; Sato, Shun ; Arai, Yoshikazu ; Tanaka, Satoshi ; Nagy, Andras ; Shiota, Kunio. / Non-CpG methylation occurs in the regulatory region of the Sry gene. In: Journal of Reproduction and Development. 2011 ; Vol. 57, No. 5. pp. 586-593.
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