Non-destructive on-chip imaging flow cell-sorting system for on-chip cellomics

Kenji Yasuda, Akihiro Hattori, Hyonchol Kim, Hideyuki Terazono, Masahito Hayashi, Hiroyuki Takei, Tomoyuki Kaneko, Fumimasa Nomura

Research output: Contribution to journalReview article

13 Citations (Scopus)

Abstract

We have developed a non-destructive imaging flow cell-sorting system using an ultra-high-speed camera (shutter speed of 1/10,000 s) with a real-time image analysis unit and a poly(methyl methacrylate) (PMMA)-based disposable microfluidic chip for single-cell-based on-chip cellomics. It has a 3-D micropipetting device that supports fully automated sorting and collection of samples. The entire fluidic system is implemented in a disposable plastic chip, enabling biological samples to be lined up in a laminar flow using hydrodynamic focusing. Its optical system enables direct observation-based cell identification using specific image indexes and phase-contrast/fluorescence microscopy, real-time image processing. It has a non-destructive, wider dynamic range, sorting procedure using mild electrostatic force in a laminar flow; agarose gel electrodes are used to prevent electrode loss and electrolysis bubble formation. The microreservoir used for recultivating collected target cells is contamination-free. An integrated ultra-high-speed droplet polymerase chain reaction measurement module is used for DNA/mRNA analysis of the collected target cells. This system was used to separate cardiomyocyte cells from a mixture of various cells. All the operations were automated using the 3-D micropipetting device. The results demonstrate that this imaging flow cell-sorting system is practically applicable for biological research and clinical diagnosis.

Original languageEnglish
Pages (from-to)907-931
Number of pages25
JournalMicrofluidics and Nanofluidics
Volume14
Issue number6
DOIs
Publication statusPublished - 2013 Jun
Externally publishedYes

Fingerprint

classifying
Sorting
chips
Imaging techniques
cells
Laminar flow
Camera shutters
Bubble formation
Electrodes
Electrostatic force
Fluorescence microscopy
Polymerase chain reaction
High speed cameras
Fluidics
Polymethyl Methacrylate
Polymethyl methacrylates
laminar flow
Electrolysis
Microfluidics
Optical systems

Keywords

  • Agarose gel microelectrode
  • Cardiomyocytes
  • Cell recultivation
  • Imaging cytometry
  • On-chip cellomics
  • On-chip imaging flow cell sorting
  • Poly(methyl methacrylate) (PMMA) disposable chip
  • Polymerase chain reaction (PCR) analysis

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Electronic, Optical and Magnetic Materials
  • Materials Chemistry

Cite this

Non-destructive on-chip imaging flow cell-sorting system for on-chip cellomics. / Yasuda, Kenji; Hattori, Akihiro; Kim, Hyonchol; Terazono, Hideyuki; Hayashi, Masahito; Takei, Hiroyuki; Kaneko, Tomoyuki; Nomura, Fumimasa.

In: Microfluidics and Nanofluidics, Vol. 14, No. 6, 06.2013, p. 907-931.

Research output: Contribution to journalReview article

Yasuda, Kenji ; Hattori, Akihiro ; Kim, Hyonchol ; Terazono, Hideyuki ; Hayashi, Masahito ; Takei, Hiroyuki ; Kaneko, Tomoyuki ; Nomura, Fumimasa. / Non-destructive on-chip imaging flow cell-sorting system for on-chip cellomics. In: Microfluidics and Nanofluidics. 2013 ; Vol. 14, No. 6. pp. 907-931.
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