Non-enzymatic in vitro production of circular hammerhead ribozyme targeting the template region of human telomerase RNA.

Akinori Ochi, S. Umekage, Yo Kikuchi

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Circular RNA is more stable than linear RNA both in vitro and in vivo because of its inaccessibility to exoribonucleases. Therefore, circularization of functional RNAs is a potentially useful methodology for designing therapeutic RNA reagents. We designed a circular hammerhead ribozyme that can cleave the template region of human telomerase RNA. This circular hammerhead ribozyme was generated by in vitro transcription followed by spontaneous self-circularization activity using the permuted intron-exon (PIE) method. Two-dimensional gel electrophoresis and alkaline digestion of the in vitro transcription products revealed that the circular hammerhead ribozyme could be produced by the PIE method. The purified circular hammerhead ribozyme cleaved the template region of human telomerase RNA in a magnesium-dependent manner. These results indicated that the circular hammerhead ribozyme generated by the PIE method maintained the specific feature of canonical hammerhead catalytic activity.

Original languageEnglish
Pages (from-to)275-276
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number53
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Introns
Exons
RNA
Exoribonucleases
Electrophoresis, Gel, Two-Dimensional
Magnesium
Digestion
In Vitro Techniques
telomerase RNA
hammerhead ribozyme
Therapeutics
circular RNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Non-enzymatic in vitro production of circular hammerhead ribozyme targeting the template region of human telomerase RNA. / Ochi, Akinori; Umekage, S.; Kikuchi, Yo.

In: Nucleic acids symposium series (2004), No. 53, 2009, p. 275-276.

Research output: Contribution to journalArticle

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