Non-genetic variability of division cycle and growth of isolated individual cells in on-chip culture system

Ippei Inoue, Yuichi Wakamoto, Kenji Yasuda

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

To investigate the non-genetic variability of the division cycle and growth of single cells, we compared pairs of Escherichia coli daughter cells' growth and division time under isolated conditions using a newly developed on-chip culture system. The advantages of the system are: (i) continuous cultivation of isolated single cells or a group of cells in μm-sized micro-cages under isolated contamination-free and exchangeable medium conditions, and (ii) continuous observation and comparison of those floating daughter cells at a spatial resolution of 0.2 μm by phase-contrast/fluorescence microscopy and digital image processing. Using this system, cell growth and division time of daughter cells from an isolated mother cell were measured. A broad distribution of the cell division time and the division time differences of two daughter cells from the same mother cells were found. They are not attributable to a difference in DNA. The same tendency of broad distribution of the division time and the division time differences suggests the involvement of a probabilistic process to start the division.

Original languageEnglish
Pages (from-to)145-150
Number of pages6
JournalProceedings of the Japan Academy Series B: Physical and Biological Sciences
Volume77
Issue number8
Publication statusPublished - 2001 Oct
Externally publishedYes

Fingerprint

division
chips
cycles
Growth
cells
Cell Division
cell division
Stem Cells
Phase-Contrast Microscopy
cell growth
Fluorescence Microscopy
digital images
fluorescence microscopy
Observation
phase contrast
Escherichia coli
Escherichia
floating
cages
image processing

Keywords

  • Cell division time
  • Cell growth speed
  • Competition of sister cells
  • Escherichia coli
  • Non-genetic variability
  • On-chip culture system
  • Single-cell culture

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • General

Cite this

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AU - Yasuda, Kenji

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N2 - To investigate the non-genetic variability of the division cycle and growth of single cells, we compared pairs of Escherichia coli daughter cells' growth and division time under isolated conditions using a newly developed on-chip culture system. The advantages of the system are: (i) continuous cultivation of isolated single cells or a group of cells in μm-sized micro-cages under isolated contamination-free and exchangeable medium conditions, and (ii) continuous observation and comparison of those floating daughter cells at a spatial resolution of 0.2 μm by phase-contrast/fluorescence microscopy and digital image processing. Using this system, cell growth and division time of daughter cells from an isolated mother cell were measured. A broad distribution of the cell division time and the division time differences of two daughter cells from the same mother cells were found. They are not attributable to a difference in DNA. The same tendency of broad distribution of the division time and the division time differences suggests the involvement of a probabilistic process to start the division.

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