Abstract
To investigate the non-genetic variability of the division cycle and growth of single cells, we compared pairs of Escherichia coli daughter cells' growth and division time under isolated conditions using a newly developed on-chip culture system. The advantages of the system are: (i) continuous cultivation of isolated single cells or a group of cells in μm-sized micro-cages under isolated contamination-free and exchangeable medium conditions, and (ii) continuous observation and comparison of those floating daughter cells at a spatial resolution of 0.2 μm by phase-contrast/fluorescence microscopy and digital image processing. Using this system, cell growth and division time of daughter cells from an isolated mother cell were measured. A broad distribution of the cell division time and the division time differences of two daughter cells from the same mother cells were found. They are not attributable to a difference in DNA. The same tendency of broad distribution of the division time and the division time differences suggests the involvement of a probabilistic process to start the division.
| Original language | English |
|---|---|
| Pages (from-to) | 145-150 |
| Number of pages | 6 |
| Journal | Proceedings of the Japan Academy Series B: Physical and Biological Sciences |
| Volume | 77 |
| Issue number | 8 |
| Publication status | Published - 2001 Oct |
| Externally published | Yes |
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Keywords
- Cell division time
- Cell growth speed
- Competition of sister cells
- Escherichia coli
- Non-genetic variability
- On-chip culture system
- Single-cell culture
ASJC Scopus subject areas
- Agricultural and Biological Sciences(all)
- General
Cite this
Non-genetic variability of division cycle and growth of isolated individual cells in on-chip culture system. / Inoue, Ippei; Wakamoto, Yuichi; Yasuda, Kenji.
In: Proceedings of the Japan Academy Series B: Physical and Biological Sciences, Vol. 77, No. 8, 10.2001, p. 145-150.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Non-genetic variability of division cycle and growth of isolated individual cells in on-chip culture system
AU - Inoue, Ippei
AU - Wakamoto, Yuichi
AU - Yasuda, Kenji
PY - 2001/10
Y1 - 2001/10
N2 - To investigate the non-genetic variability of the division cycle and growth of single cells, we compared pairs of Escherichia coli daughter cells' growth and division time under isolated conditions using a newly developed on-chip culture system. The advantages of the system are: (i) continuous cultivation of isolated single cells or a group of cells in μm-sized micro-cages under isolated contamination-free and exchangeable medium conditions, and (ii) continuous observation and comparison of those floating daughter cells at a spatial resolution of 0.2 μm by phase-contrast/fluorescence microscopy and digital image processing. Using this system, cell growth and division time of daughter cells from an isolated mother cell were measured. A broad distribution of the cell division time and the division time differences of two daughter cells from the same mother cells were found. They are not attributable to a difference in DNA. The same tendency of broad distribution of the division time and the division time differences suggests the involvement of a probabilistic process to start the division.
AB - To investigate the non-genetic variability of the division cycle and growth of single cells, we compared pairs of Escherichia coli daughter cells' growth and division time under isolated conditions using a newly developed on-chip culture system. The advantages of the system are: (i) continuous cultivation of isolated single cells or a group of cells in μm-sized micro-cages under isolated contamination-free and exchangeable medium conditions, and (ii) continuous observation and comparison of those floating daughter cells at a spatial resolution of 0.2 μm by phase-contrast/fluorescence microscopy and digital image processing. Using this system, cell growth and division time of daughter cells from an isolated mother cell were measured. A broad distribution of the cell division time and the division time differences of two daughter cells from the same mother cells were found. They are not attributable to a difference in DNA. The same tendency of broad distribution of the division time and the division time differences suggests the involvement of a probabilistic process to start the division.
KW - Cell division time
KW - Cell growth speed
KW - Competition of sister cells
KW - Escherichia coli
KW - Non-genetic variability
KW - On-chip culture system
KW - Single-cell culture
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UR - http://www.scopus.com/inward/citedby.url?scp=0042905293&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0042905293
VL - 77
SP - 145
EP - 150
JO - Proceedings of the Japan Academy Series B: Physical and Biological Sciences
JF - Proceedings of the Japan Academy Series B: Physical and Biological Sciences
SN - 0386-2208
IS - 8
ER -