Non-invasive Blood Glucose Measurement (NGM) technology is a crucial challenge for not only academic communities but also industrial communities. Therefore, finding an applicable and accurate NGM technology is supportive and desired for patients with hyperglycemia or hypoglycemia. Among various NGM technologies, infrared is considered as the most popular and prospective method. In this paper, the mid-infrared spectroscope is used in the experiment, there are significant differences in transmittance in the range of 3000 3500 cm-1 (3333 2857 nm). The association between mid-infrared spectroscopy transmittance and blood glucose concentration is presented. The infrared with a peak wavelength of 3000 3500 cm-1could be a good solution to measuring the physical signals in the experiment. Besides, an NGM prototype that aims to improve self-management motivation is designed and presented. Mid-infrared is very potential and prospective in NGM research. Also, further investigation and consideration ear needed in future work.