Non-universal decoding of the leucine codon CUG in several Candida species

Takeshi Ohama, Tsutomu Suzuki, Miki Mori, Syozo Osawa, Takuya Ueda*, Kimitsuna Watanabe, Takashi Nakase

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

143 Citations (Scopus)


It has been reported that CUG, a universal leucine codon, is read as serine In an asporogenlc yeast, Candida cyllndracea. The distribution of this non-universal genetic code in various yeast species was studied using an In vitro translation assay system with a synthetic messenger RNA containing CUG codons in-frame. It was found that CUG is used as a serine codon in six out of the fourteen species examined, while it is used for leucine in the remaining eight. The tRNA species responsible for the translation of codon CUG as serine was detected in all the six species in which CUG is translated as serine. The grouping according to the CUG codon assignments in these yeast species shows a good correlation with physiological classification by the chain lengths of the Isoprenold moiety of ublquinone and the cell-wall sugar contained in the yeasts. The six Candida species examined in which CUG is used as serine belong to one distinct group in Hemlascomycetes.

Original languageEnglish
Pages (from-to)4039-4045
Number of pages7
JournalNucleic acids research
Issue number17
Publication statusPublished - 1993 Aug 25
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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