Nonlinear force-length relationship in the ADP-induced contraction of skeletal myofibrils

Yuta Shimamoto, Fumiaki Kono, Madoka Suzuki, Shin'ichi Ishiwata

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The regulatory mechanism of sarcomeric activity has not been fully clarified yet because of its complex and cooperative nature, which involves both Ca2+ and cross-bridge binding to the thin filament. To reveal the mechanism of regulation mediated by the cross-bridges, separately from the effect of Ca2+, we investigated the force-sarcomere length (SL) relationship in rabbit skeletal myofibrils (a single myofibril or a thin bundle) at SL > 2.2 μm in the absence of Ca2+ at various levels of activation by exogenous MgADP (4-20 mM) in the presence of 1 mM MgATP. The individual SLs were measured by phase-contrast microscopy to confirm the homogeneity of the striation pattern of sarcomeres during activation. We found that at partial activation with 4-8 mM MgADP, the developed force nonlinearly depended on the length of overlap between the thick and the thin filaments; that is, contrary to the maximal activation, the maximal active force was generated at shorter overlap. Besides, the active force became larger, whereas this nonlinearity tended to weaken, with either an increase in [MgADP] or the lateral osmotic compression of the myofilament lattice induced by the addition of a macromolecular compound, dextran T-500. The model analysis, which takes into account the [MgADP]- and the lattice-spacing-dependent probability of cross-bridge formation, was successfully applied to account for the force-SL relationship observed at partial activation. These results strongly suggest that the cross-bridge works as a cooperative activator, the function of which is highly sensitive to as little as ≤ 1 nm changes in the lattice spacing.

Original languageEnglish
Pages (from-to)4330-4341
Number of pages12
JournalBiophysical Journal
Volume93
Issue number12
DOIs
Publication statusPublished - 2007 Dec 15

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Sarcomeres
Myofibrils
Adenosine Diphosphate
Activator Appliances
Macromolecular Substances
Phase-Contrast Microscopy
Dextrans
Adenosine Triphosphate
Rabbits

ASJC Scopus subject areas

  • Biophysics

Cite this

Nonlinear force-length relationship in the ADP-induced contraction of skeletal myofibrils. / Shimamoto, Yuta; Kono, Fumiaki; Suzuki, Madoka; Ishiwata, Shin'ichi.

In: Biophysical Journal, Vol. 93, No. 12, 15.12.2007, p. 4330-4341.

Research output: Contribution to journalArticle

Shimamoto, Yuta ; Kono, Fumiaki ; Suzuki, Madoka ; Ishiwata, Shin'ichi. / Nonlinear force-length relationship in the ADP-induced contraction of skeletal myofibrils. In: Biophysical Journal. 2007 ; Vol. 93, No. 12. pp. 4330-4341.
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