Nonphotic entrainment by 5-HT(1a/7) receptor agonists accompanied by reduced Per1 and Per2 mRNA levels in the suprachiasmatic nuclei

Kazumasa Horikawa, Shin Ichi Yokota, Kazuyuki Fuji, Masashi Akiyama, Takahiro Moriya, Hitoshi Okamura, Shigenobu Shibata

    Research output: Contribution to journalArticle

    159 Citations (Scopus)

    Abstract

    In mammals, the environmental light/dark cycle strongly synchronizes the circadian clock within the suprachiasmatic nuclei (SCN) to 24 hr. It is well known that not only photic but also nonphotic stimuli can entrain the SCN clock. Actually, many studies have shown that a daytime injection of 8-hydroxy-2-(di-n-propylamino) tetralin (8-OH DPAT), a serotonin 1A/7 receptor agonist, as a nonphotic stimulus induces phase advances in hamster behavioral circadian rhythms in vivo, as well as the neuron activity rhythm of the SCN in vitro. Recent reports suggest that mammalian homologs of the Drosophila clock gene, Period (Per), are involved in photic entrainment. Therefore, we examined whether phase advances elicited by 8-OH DPAT were associated with a change of Period mRNA levels in the SCN. In this experiment, we cloned partial cDNAs encoding hamster Per1, Per2, and Per3 and observed both circadian oscillation and the light responsiveness of Period. Furthermore, we found that the inhibitory effect of 8-OH DPAT on hamster Per1 and Per2 mRNA levels in the SCN occurred only during the hamster's mid-subjective day, but not during the early subjective day or subjective night. The present findings demonstrate that the acute and circadian time-dependent reduction of Per1 and/or Per2 mRNA in the hamster SCN by 8-OH DPAT is strongly correlated with the phase resetting in response to 8-OH DPAT.

    Original languageEnglish
    Pages (from-to)5867-5873
    Number of pages7
    JournalJournal of Neuroscience
    Volume20
    Issue number15
    Publication statusPublished - 2000 Aug 1

    Fingerprint

    8-Hydroxy-2-(di-n-propylamino)tetralin
    Suprachiasmatic Nucleus
    Receptor, Serotonin, 5-HT1A
    Cricetinae
    Messenger RNA
    Circadian Clocks
    Photoperiod
    Circadian Rhythm
    Drosophila
    Mammals
    Complementary DNA
    Neurons
    Light
    Injections
    Genes

    Keywords

    • 5-HT(1A/7) receptor
    • 8-OH DPAT
    • Circadian rhythm
    • Hamster
    • NIH Image
    • Per mRNA
    • Suprachiasmatic nucleus

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Nonphotic entrainment by 5-HT(1a/7) receptor agonists accompanied by reduced Per1 and Per2 mRNA levels in the suprachiasmatic nuclei. / Horikawa, Kazumasa; Yokota, Shin Ichi; Fuji, Kazuyuki; Akiyama, Masashi; Moriya, Takahiro; Okamura, Hitoshi; Shibata, Shigenobu.

    In: Journal of Neuroscience, Vol. 20, No. 15, 01.08.2000, p. 5867-5873.

    Research output: Contribution to journalArticle

    Horikawa, Kazumasa ; Yokota, Shin Ichi ; Fuji, Kazuyuki ; Akiyama, Masashi ; Moriya, Takahiro ; Okamura, Hitoshi ; Shibata, Shigenobu. / Nonphotic entrainment by 5-HT(1a/7) receptor agonists accompanied by reduced Per1 and Per2 mRNA levels in the suprachiasmatic nuclei. In: Journal of Neuroscience. 2000 ; Vol. 20, No. 15. pp. 5867-5873.
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    AU - Fuji, Kazuyuki

    AU - Akiyama, Masashi

    AU - Moriya, Takahiro

    AU - Okamura, Hitoshi

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