Noradrenergic modulation of gonadotrophin-inhibitory hormone gene expression in the brain of Japanese quail

Y. Tobari, N. Kansaku, Kazuyoshi Tsutsui

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Gonadotrophin-inhibitory hormone (GnIH) is a hypothalamic neuropeptide that inhibits gonadotrophin synthesis and release in birds and mammals. In Japanese quail, GnIH neurones express the noradrenergic receptor and receive noradrenergic innervation. Treatment with noradrenaline (NA) stimulates GnIH release from diencephalic tissue blocks in vitro. However, the effects of NA on hypothalamic GnIH gene expression have not been determined. We investigated noradrenergic regulation of GnIH gene expression in the brain of male quail using the selective noradrenergic neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP-4). We first showed that DSP-4 reduced the number of noradrenergic (dopamine-β-hydroxylase immunoreactive) cells in the locus coeruleus (LoC) and specifically lowered the NA concentration in the hypothalamus of male quail. Other monoamines, such as dopamine and serotonin, were not affected by drug treatment. DSP-4 did not decrease the numbers of noradrenergic cells of the lateral tegmental cell group, nor the plasma NA concentration. Decreased hypothalamic NA levels after DSP-4 treatment did not change GnIH gene expression in the brains of quail during their interaction with conspecifics. On the other hand, GnIH gene expression increased in the brains of quail socially isolated for 1 hour after DSP-4 treatment. These results suggest that some noradrenergic neurones have inhibitory effects on GnIH gene expression of the hypothalamus in solitary quail.

    Original languageEnglish
    Article numbere12503
    JournalJournal of Neuroendocrinology
    Volume29
    Issue number8
    DOIs
    Publication statusPublished - 2017 Aug 1

    Fingerprint

    Coturnix
    Gonadotropins
    Hormones
    Quail
    Gene Expression
    Brain
    Norepinephrine
    Adrenergic Neurons
    Hypothalamus
    Dopamine
    Locus Coeruleus
    Neurotoxins
    Mixed Function Oxygenases
    Neuropeptides
    Birds
    DSP 4
    Mammals
    Serotonin
    Cell Count

    Keywords

    • monoamine
    • N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine hydrochloride (DSP-4)
    • neuropeptide
    • norepinephrine
    • RFamide-related peptide (RFRP)
    • social isolation

    ASJC Scopus subject areas

    • Endocrinology, Diabetes and Metabolism
    • Endocrinology
    • Endocrine and Autonomic Systems
    • Cellular and Molecular Neuroscience

    Cite this

    Noradrenergic modulation of gonadotrophin-inhibitory hormone gene expression in the brain of Japanese quail. / Tobari, Y.; Kansaku, N.; Tsutsui, Kazuyoshi.

    In: Journal of Neuroendocrinology, Vol. 29, No. 8, e12503, 01.08.2017.

    Research output: Contribution to journalArticle

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