Novel device for transplantation of cell sheet and evaluation of thin polymer films by atomic force microscopy

Ryohei Takeuchi, Kazuhiro Fukumori, Katsuhisa Sakaguchi, Yutaka Terajima, Tatsuya Shimizu, Teruo Okano, Mitsuo Umezu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Cell therapy is expected to a new tool to treat refractory diseases. In heart regeneration, it has been firstly conducted with needle injection of cell suspensions. Recently, cell sheet engineering emerged as another method of cell therapy. Cell sheet is prepared with a temperature responsive dish by temperature reduction. It is a thin-patch-like tissue construct and its thickness is several tens of micrometers. It is composed of cells and intrinsic extra cellular matrix only. The transplantation of the cell sheet has been already conducted in animal experiments and even in clinical trials. The cell sheet is transplanted at the surface of the heart, but it is difficult to transplant the cell sheet under the beating heart. To overcome this difficulty, we designed a device that was composed of two thin polymer films that have different friction. The films were made of polyurethane, polyethylene, or polypropylene. The cell sheet was set up on the device by sandwiching it with the less frictional film and the more frictional film. In this paper, using two different films having the different friction, the cell sheet was successfully transplanted to the static round polymer surface, the harvested heart, and even the beating heart of pig by removing the films step by step using the difference in friction. Also, surface properties such as friction, adhesion force and roughness of the films were studied by an atomic force microscopy (AFM). From the results of the study, the friction of the film was found to be likely proportional to the adhesion force and the inverse of roughness.

Original languageEnglish
Title of host publication2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
Pages168-173
Number of pages6
DOIs
Publication statusPublished - 2012
Event22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation - Nagoya
Duration: 2011 Nov 62011 Nov 9

Other

Other22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
CityNagoya
Period11/11/611/11/9

Fingerprint

Polymer films
Atomic force microscopy
Thin films
Friction
Adhesion
Surface roughness
Transplants
Needles
Refractory materials
Polyurethanes
Surface properties
Polyethylenes
Polypropylenes
Animals
Tissue
Temperature
Polymers
Experiments

ASJC Scopus subject areas

  • Artificial Intelligence
  • Mechanical Engineering

Cite this

Takeuchi, R., Fukumori, K., Sakaguchi, K., Terajima, Y., Shimizu, T., Okano, T., & Umezu, M. (2012). Novel device for transplantation of cell sheet and evaluation of thin polymer films by atomic force microscopy. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation" (pp. 168-173). [6102201] https://doi.org/10.1109/MHS.2011.6102201

Novel device for transplantation of cell sheet and evaluation of thin polymer films by atomic force microscopy. / Takeuchi, Ryohei; Fukumori, Kazuhiro; Sakaguchi, Katsuhisa; Terajima, Yutaka; Shimizu, Tatsuya; Okano, Teruo; Umezu, Mitsuo.

2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. p. 168-173 6102201.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Takeuchi, R, Fukumori, K, Sakaguchi, K, Terajima, Y, Shimizu, T, Okano, T & Umezu, M 2012, Novel device for transplantation of cell sheet and evaluation of thin polymer films by atomic force microscopy. in 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"., 6102201, pp. 168-173, 22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation, Nagoya, 11/11/6. https://doi.org/10.1109/MHS.2011.6102201
Takeuchi R, Fukumori K, Sakaguchi K, Terajima Y, Shimizu T, Okano T et al. Novel device for transplantation of cell sheet and evaluation of thin polymer films by atomic force microscopy. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. p. 168-173. 6102201 https://doi.org/10.1109/MHS.2011.6102201
Takeuchi, Ryohei ; Fukumori, Kazuhiro ; Sakaguchi, Katsuhisa ; Terajima, Yutaka ; Shimizu, Tatsuya ; Okano, Teruo ; Umezu, Mitsuo. / Novel device for transplantation of cell sheet and evaluation of thin polymer films by atomic force microscopy. 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation". 2012. pp. 168-173
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