Novel fish hypothalamic neuropeptides stimulate the release of gonadotrophins and growth hormone from the pituitary of sockeye salmon

Masafumi Amano, Shunsuke Moriyama, Masayuki Ligo, Shoji Kitamura, Noriko Amiya, Kunio Yamamori, Kazuyoshi Ukena, Kazuyoshi Tsutsui

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

We recently identified a cDNA encoding three novel fish hypothalamic neuropeptides, having LPXRF-NH2 from the goldfish brain. In this study, to clarify the physiological functions of these three LPXRFamide peptides (gfLPXRFa-1, -2, and -3), we analysed the localisation and hypophysiotrophic activity of these peptides using sockeye salmon, Oncorhynchus nerka, in which immunoassay systems for several anterior pituitary hormones have been developed. gfLPXRFa-immunoreactive cell bodies were detected in the nucleus posterioris periventricularis of the hypothalamus and immunoreactive fibres were distributed in various brain regions and the pituitary. We also detected gfLPXRFa-immunoreactivity in the pituitary by competitive enzyme-linked immunosorbent assay combined with reversed-phase HPLC. These three gfLPXRFamide peptides stimulated the release of FSH, LH and GH, but did not affect the release of prolactin (PRL) and somatolactin (SL) from cultured pituitary cells. These results suggest that novel fish hypothalamic LPXR-Famide peptides exist in the brain and pituitary of sockeye salmon and stimulate the release of gonadotrophins and GH from the pituitary.

Original languageEnglish
Pages (from-to)417-423
Number of pages7
JournalJournal of Endocrinology
Volume188
Issue number3
DOIs
Publication statusPublished - 2006 Mar
Externally publishedYes

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Salmon
Neuropeptides
Gonadotropins
Growth Hormone
Fishes
Peptides
Brain
Anterior Pituitary Hormones
Pituitary Gonadotropins
Goldfish
Immunoassay
Prolactin
Hypothalamus
Cultured Cells
Complementary DNA
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Endocrinology

Cite this

Novel fish hypothalamic neuropeptides stimulate the release of gonadotrophins and growth hormone from the pituitary of sockeye salmon. / Amano, Masafumi; Moriyama, Shunsuke; Ligo, Masayuki; Kitamura, Shoji; Amiya, Noriko; Yamamori, Kunio; Ukena, Kazuyoshi; Tsutsui, Kazuyoshi.

In: Journal of Endocrinology, Vol. 188, No. 3, 03.2006, p. 417-423.

Research output: Contribution to journalArticle

Amano, Masafumi ; Moriyama, Shunsuke ; Ligo, Masayuki ; Kitamura, Shoji ; Amiya, Noriko ; Yamamori, Kunio ; Ukena, Kazuyoshi ; Tsutsui, Kazuyoshi. / Novel fish hypothalamic neuropeptides stimulate the release of gonadotrophins and growth hormone from the pituitary of sockeye salmon. In: Journal of Endocrinology. 2006 ; Vol. 188, No. 3. pp. 417-423.
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