Novel metabolic pathway for salicylate biodegradation via phenol in yeast Trichosporon moniliiforme

Yuichiro Iwasaki; Hiroaki Gunji; Kuniki Kino; Takasumi Hattori; Yoshitaka Ishii; Kohtaro Kirimura

doi:10.1007/s10532-009-9323-6

Novel metabolic pathway for salicylate biodegradation via phenol in yeast Trichosporon moniliiforme

Yuichiro Iwasaki, Hiroaki Gunji, Kuniki Kino, Takasumi Hattori, Yoshitaka Ishii, Kohtaro Kirimura^*

^*Corresponding author for this work

School of Advanced Science and Engineering

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

A novel metabolic pathway was found in the yeast Trichosporon moniliiforme WU-0401 for salicylate degradation via phenol as the key intermediate. When 20 mM salicylate was used as the sole carbon source for the growth of strain WU-0401, phenol was detected as a distinct metabolite in the culture broth. Analysis of the products derived from salicylate or phenol through reactions with resting cells and a cell-free extract of strain WU-0401 indicated that salicylate is initially decarboxylated to phenol and then oxidized to catechol, followed by aromatic ring cleavage to form cis-cis muconate.

Original language	English
Pages (from-to)	557-564
Number of pages	8
Journal	Biodegradation
Volume	21
Issue number	4
DOIs	https://doi.org/10.1007/s10532-009-9323-6
Publication status	Published - 2010 Jul

Keywords

Biodegradation
Metabolic pathway
Phenol
Salicylate
Trichosporon moniliiforme

ASJC Scopus subject areas

Environmental Engineering
Microbiology
Bioengineering
Environmental Chemistry
Pollution

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10.1007/s10532-009-9323-6

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title = "Novel metabolic pathway for salicylate biodegradation via phenol in yeast Trichosporon moniliiforme",

abstract = "A novel metabolic pathway was found in the yeast Trichosporon moniliiforme WU-0401 for salicylate degradation via phenol as the key intermediate. When 20 mM salicylate was used as the sole carbon source for the growth of strain WU-0401, phenol was detected as a distinct metabolite in the culture broth. Analysis of the products derived from salicylate or phenol through reactions with resting cells and a cell-free extract of strain WU-0401 indicated that salicylate is initially decarboxylated to phenol and then oxidized to catechol, followed by aromatic ring cleavage to form cis-cis muconate.",

keywords = "Biodegradation, Metabolic pathway, Phenol, Salicylate, Trichosporon moniliiforme",

author = "Yuichiro Iwasaki and Hiroaki Gunji and Kuniki Kino and Takasumi Hattori and Yoshitaka Ishii and Kohtaro Kirimura",

note = "Funding Information: Acknowledgments This study was supported in part by the Global-COE Program {\textquoteleft}{\textquoteleft}Center for Practical Chemical Wisdom{\textquoteright}{\textquoteright} from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan and by the Waseda University Grant for Special Research Projects.",

year = "2010",

month = jul,

doi = "10.1007/s10532-009-9323-6",

language = "English",

volume = "21",

pages = "557--564",

journal = "Biodegradation",

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publisher = "Springer Netherlands",

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T1 - Novel metabolic pathway for salicylate biodegradation via phenol in yeast Trichosporon moniliiforme

AU - Iwasaki, Yuichiro

AU - Gunji, Hiroaki

AU - Kino, Kuniki

AU - Hattori, Takasumi

AU - Ishii, Yoshitaka

AU - Kirimura, Kohtaro

N1 - Funding Information: Acknowledgments This study was supported in part by the Global-COE Program ‘‘Center for Practical Chemical Wisdom’’ from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan and by the Waseda University Grant for Special Research Projects.

PY - 2010/7

Y1 - 2010/7

N2 - A novel metabolic pathway was found in the yeast Trichosporon moniliiforme WU-0401 for salicylate degradation via phenol as the key intermediate. When 20 mM salicylate was used as the sole carbon source for the growth of strain WU-0401, phenol was detected as a distinct metabolite in the culture broth. Analysis of the products derived from salicylate or phenol through reactions with resting cells and a cell-free extract of strain WU-0401 indicated that salicylate is initially decarboxylated to phenol and then oxidized to catechol, followed by aromatic ring cleavage to form cis-cis muconate.

AB - A novel metabolic pathway was found in the yeast Trichosporon moniliiforme WU-0401 for salicylate degradation via phenol as the key intermediate. When 20 mM salicylate was used as the sole carbon source for the growth of strain WU-0401, phenol was detected as a distinct metabolite in the culture broth. Analysis of the products derived from salicylate or phenol through reactions with resting cells and a cell-free extract of strain WU-0401 indicated that salicylate is initially decarboxylated to phenol and then oxidized to catechol, followed by aromatic ring cleavage to form cis-cis muconate.

KW - Biodegradation

KW - Metabolic pathway

KW - Phenol

KW - Salicylate

KW - Trichosporon moniliiforme

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DO - 10.1007/s10532-009-9323-6

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SN - 0923-9820

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JO - Biodegradation

JF - Biodegradation

IS - 4

ER -

Novel metabolic pathway for salicylate biodegradation via phenol in yeast Trichosporon moniliiforme

Abstract

Keywords

ASJC Scopus subject areas

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