Novel method for selection of antimicrobial peptides from a phage display library by use of bacterial magnetic particles

Tsuyoshi Tanaka, Yoriko Kokuryu, Tadashi Matsunaga

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Antimicrobial peptides were isolated from a phage display peptide library using bacterial magnetic particles (BacMPs) as a solid support. The BacMPs obtained from "Magnetospirillum magneticum" strain AMB-1 consist of pure magnetite (50 to 100 nm in size) and are covered with a lipid bilayer membrane derived from the invagination of the inner membrane. BacMPs are easily purified from a culture of magnetotactic bacteria by magnetic separation. Approximately 4 × 1010 PFU of the library phage (complexity, 2.7 × 109) was reacted with BacMPs. The elution of bound phages from BacMPs was performed by disrupting its membrane with phospholipase D treatment. Six candidate peptides, which were highly cationic and could bind onto the BacMP membrane, were obtained. They exhibited antimicrobial activity against Bacillus subtilis but not against Escherichia coli and Saccharomyces cerevisiae. The amino acid substitution of the selected peptide, KPQQHN RPLRHK (peptide 6-7), to enhance the hydrophobicity resulted in obvious antimicrobial activity against all test microorganisms. The present study shows for the first time that a magnetic selection of antimicrobial peptides from the phage display peptide library was successfully achieved by targeting the actual bacterial inner membrane. This BacMP-based method could be a promising approach for a high-throughput screening of antimicrobial peptides targeting a wide range of species.

Original languageEnglish
Pages (from-to)7600-7606
Number of pages7
JournalApplied and Environmental Microbiology
Volume74
Issue number24
DOIs
Publication statusPublished - 2008 Dec
Externally publishedYes

Fingerprint

Peptide Library
antimicrobial peptides
bacteriophages
peptide
Peptides
peptide libraries
Membranes
membrane
peptides
Magnetospirillum
Bacteriophages
anti-infective agents
magnetic separation
antimicrobial activity
magnetite
phospholipase D
methodology
lipid bilayers
Ferrosoferric Oxide
DNA libraries

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Novel method for selection of antimicrobial peptides from a phage display library by use of bacterial magnetic particles. / Tanaka, Tsuyoshi; Kokuryu, Yoriko; Matsunaga, Tadashi.

In: Applied and Environmental Microbiology, Vol. 74, No. 24, 12.2008, p. 7600-7606.

Research output: Contribution to journalArticle

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