Novel monofunctional substrates of polynucleotide phosphorylase: The "single-addition" of 2′(3′)-o-dihydrocinnamoyl-nucleoside 5′-diphosphate to a primer oligonucleotide

Yo Kikuchi, Keiko Hirai, Kenji Sakaguchi

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15 Citations (Scopus)

Abstract

A method was developed for stepwise synthesis of oligonucleotides of denned sequence using 2′(3′)-O-dihydrocinnamoyl-nucleoside 5′-diphosphates as substrates for polynucleotide phosphorylase [EC 2.7.7.8]. Polynucleotide phosphorylase from Thermus thermophilus catalyzed the transfer of one 2′(3′)-O-dihydrocinnamoyl-adenylate residue from 2′(3′)-blocked ADP to the 3′-terminus of the primer trinucleoside diphosphate, ApApA. The product was 2′(3′)-substituted triadenylyladenosine. The blocking group, dihydrocinnamoyl, could be removed completely from the product without destruction of the phosphodiester bond using α-chymotrypsin [EC 3.4.21.1] at neutral pH.

Original languageEnglish
Pages (from-to)469-472
Number of pages4
JournalJournal of Biochemistry
Volume77
Issue number2
Publication statusPublished - 1975 Feb
Externally publishedYes

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ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

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