Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic Dictyostelium cells

Akira Nagasaki, Go Itoh, Shigehiko Yumura, Taro Q.P. Uyeda

Research output: Contribution to journalArticlepeer-review

22 Citations (Scopus)

Abstract

We have cloned a full-length cDNA encoding a novel myosin II heavy chain kinase (mhckC) from Dictyostelium. Like other members of the myosin heavy chain kinase family, the mhckC gene product, MHCK C, has a kinase domain in its N-terminal half and six WD repeats in the C-terminal half. GFP-MHCK C fusion protein localized to the cortex of interphase cells, to the cleavage furrow of mitotic cells, and to the posterior of migrating cells. These distributions of GFP-MHCK C always corresponded with that of myosin II filaments and were not observed in myosin II-null cells, where GFP-MHCK C was diffusely distributed in the cytoplasm. Thus, localization of MHCK C seems to be myosin II-dependent. Cells lacking the mhckC gene exhibited excessive aggregation of myosin II filaments in the cleavage furrows and in the posteriors of the daughter cells once cleavage was complete. The cleavage process of these cells took longer than that of wild-type cells. Taken together, these findings suggest MHCK C drives the disassembly of myosin II filaments for efficient cytokinesis and recycling of myosin II that occurs during cytokinesis.

Original languageEnglish
Pages (from-to)4333-4342
Number of pages10
JournalMolecular biology of the cell
Volume13
Issue number12
DOIs
Publication statusPublished - 2002 Dec 1
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Novel myosin heavy chain kinase involved in disassembly of myosin II filaments and efficient cleavage in mitotic Dictyostelium cells'. Together they form a unique fingerprint.

Cite this