Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells

Yoshiaki Maeda, Tomoko Yoshino, Tadashi Matsunaga

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Novel nanocomposites consisting of nano-sized bacterial magnetic particles (BacMPs) and semiconductor quantum dots (QD) were developed for use in targeting and identifying cancer cells. BacMPs are synthesized by magnetotactic bacterium, Magnetospirillum magneticum AMB-1 and can display functional proteins using gene fusion techniques. In this study, two functional proteins, biotin carboxyl carrier protein (BCCP), derived from AMB-1 and protein G, derived from Streptococcus were displayed on BacMPs. BCCP was biotinylated in AMB-1 cells by endogenous biotin ligase. After purification of in vivo-biotinylated BacMPs, streptavidin and antibodies were immobilized on protein G-BCCP-displaying BacMPs (protein G-BCCP-BacMPs) via biotin and protein G, respectively. Furthermore, multi-color labeling of the protein G-BCCP-BacMPs was achieved with streptavidin-labeled QD. Using streptavidin-QD/protein G-BCCP-BacMP nanocomposites, we successfully demonstrated magnetic manipulation and fluorescent labeling of lung cancer cells.

Original languageEnglish
Pages (from-to)6361-6366
Number of pages6
JournalJournal of Materials Chemistry
Volume19
Issue number35
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Magnetic separation
Labeling
Semiconductor quantum dots
Nanocomposites
Cells
Proteins
Streptavidin
Biotin
Ligases
Purification
biotin carboxyl carrier protein
Antibodies
Bacteria
Fusion reactions
Carrier Proteins
Color
Genes

ASJC Scopus subject areas

  • Materials Chemistry
  • Chemistry(all)

Cite this

Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells. / Maeda, Yoshiaki; Yoshino, Tomoko; Matsunaga, Tadashi.

In: Journal of Materials Chemistry, Vol. 19, No. 35, 2009, p. 6361-6366.

Research output: Contribution to journalArticle

@article{a0ecb6e01ba84bafb10cd27564034b31,
title = "Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells",
abstract = "Novel nanocomposites consisting of nano-sized bacterial magnetic particles (BacMPs) and semiconductor quantum dots (QD) were developed for use in targeting and identifying cancer cells. BacMPs are synthesized by magnetotactic bacterium, Magnetospirillum magneticum AMB-1 and can display functional proteins using gene fusion techniques. In this study, two functional proteins, biotin carboxyl carrier protein (BCCP), derived from AMB-1 and protein G, derived from Streptococcus were displayed on BacMPs. BCCP was biotinylated in AMB-1 cells by endogenous biotin ligase. After purification of in vivo-biotinylated BacMPs, streptavidin and antibodies were immobilized on protein G-BCCP-displaying BacMPs (protein G-BCCP-BacMPs) via biotin and protein G, respectively. Furthermore, multi-color labeling of the protein G-BCCP-BacMPs was achieved with streptavidin-labeled QD. Using streptavidin-QD/protein G-BCCP-BacMP nanocomposites, we successfully demonstrated magnetic manipulation and fluorescent labeling of lung cancer cells.",
author = "Yoshiaki Maeda and Tomoko Yoshino and Tadashi Matsunaga",
year = "2009",
doi = "10.1039/b900693a",
language = "English",
volume = "19",
pages = "6361--6366",
journal = "Journal of Materials Chemistry",
issn = "0959-9428",
publisher = "Royal Society of Chemistry",
number = "35",

}

TY - JOUR

T1 - Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells

AU - Maeda, Yoshiaki

AU - Yoshino, Tomoko

AU - Matsunaga, Tadashi

PY - 2009

Y1 - 2009

N2 - Novel nanocomposites consisting of nano-sized bacterial magnetic particles (BacMPs) and semiconductor quantum dots (QD) were developed for use in targeting and identifying cancer cells. BacMPs are synthesized by magnetotactic bacterium, Magnetospirillum magneticum AMB-1 and can display functional proteins using gene fusion techniques. In this study, two functional proteins, biotin carboxyl carrier protein (BCCP), derived from AMB-1 and protein G, derived from Streptococcus were displayed on BacMPs. BCCP was biotinylated in AMB-1 cells by endogenous biotin ligase. After purification of in vivo-biotinylated BacMPs, streptavidin and antibodies were immobilized on protein G-BCCP-displaying BacMPs (protein G-BCCP-BacMPs) via biotin and protein G, respectively. Furthermore, multi-color labeling of the protein G-BCCP-BacMPs was achieved with streptavidin-labeled QD. Using streptavidin-QD/protein G-BCCP-BacMP nanocomposites, we successfully demonstrated magnetic manipulation and fluorescent labeling of lung cancer cells.

AB - Novel nanocomposites consisting of nano-sized bacterial magnetic particles (BacMPs) and semiconductor quantum dots (QD) were developed for use in targeting and identifying cancer cells. BacMPs are synthesized by magnetotactic bacterium, Magnetospirillum magneticum AMB-1 and can display functional proteins using gene fusion techniques. In this study, two functional proteins, biotin carboxyl carrier protein (BCCP), derived from AMB-1 and protein G, derived from Streptococcus were displayed on BacMPs. BCCP was biotinylated in AMB-1 cells by endogenous biotin ligase. After purification of in vivo-biotinylated BacMPs, streptavidin and antibodies were immobilized on protein G-BCCP-displaying BacMPs (protein G-BCCP-BacMPs) via biotin and protein G, respectively. Furthermore, multi-color labeling of the protein G-BCCP-BacMPs was achieved with streptavidin-labeled QD. Using streptavidin-QD/protein G-BCCP-BacMP nanocomposites, we successfully demonstrated magnetic manipulation and fluorescent labeling of lung cancer cells.

UR - http://www.scopus.com/inward/record.url?scp=69249197820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69249197820&partnerID=8YFLogxK

U2 - 10.1039/b900693a

DO - 10.1039/b900693a

M3 - Article

AN - SCOPUS:69249197820

VL - 19

SP - 6361

EP - 6366

JO - Journal of Materials Chemistry

JF - Journal of Materials Chemistry

SN - 0959-9428

IS - 35

ER -