Novel nanocomposites consisting of in vivo-biotinylated bacterial magnetic particles and quantum dots for magnetic separation and fluorescent labeling of cancer cells

Yoshiaki Maeda, Tomoko Yoshino, Tadashi Matsunaga

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Novel nanocomposites consisting of nano-sized bacterial magnetic particles (BacMPs) and semiconductor quantum dots (QD) were developed for use in targeting and identifying cancer cells. BacMPs are synthesized by magnetotactic bacterium, Magnetospirillum magneticum AMB-1 and can display functional proteins using gene fusion techniques. In this study, two functional proteins, biotin carboxyl carrier protein (BCCP), derived from AMB-1 and protein G, derived from Streptococcus were displayed on BacMPs. BCCP was biotinylated in AMB-1 cells by endogenous biotin ligase. After purification of in vivo-biotinylated BacMPs, streptavidin and antibodies were immobilized on protein G-BCCP-displaying BacMPs (protein G-BCCP-BacMPs) via biotin and protein G, respectively. Furthermore, multi-color labeling of the protein G-BCCP-BacMPs was achieved with streptavidin-labeled QD. Using streptavidin-QD/protein G-BCCP-BacMP nanocomposites, we successfully demonstrated magnetic manipulation and fluorescent labeling of lung cancer cells.

Original languageEnglish
Pages (from-to)6361-6366
Number of pages6
JournalJournal of Materials Chemistry
Volume19
Issue number35
DOIs
Publication statusPublished - 2009
Externally publishedYes

ASJC Scopus subject areas

  • Materials Chemistry
  • Chemistry(all)

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