Novel nitritation process using heat-shocked nitrifying bacteria entrapped in gel carriers

Kazuichi Isaka, Tatsuo Sumino, Satoshi Tsuneda

    Research output: Contribution to journalArticle

    25 Citations (Scopus)

    Abstract

    Nitritation (ammonium being oxidized to nitrite) is a cost-effective method for treating wastewater having high ammonium concentrations or low C/N ratios. We developed a novel nitritation process based on the observation that nitrite-oxidizing bacteria (NOB) in sewage sludge can be killed by heat shock, but ammonium-oxidizing bacteria (AOB) may survive. The effects of maximum heat-shock temperature and heat-shock duration on populations of AOB and NOB in gel carriers were measured. No NOB were detected after a heat-shock treatment higher than 60 °C for 20 min. However, the population of AOB continued to exist at above 108 MPN/mL-carrier even after heat shock at 80 °C for 1 h. To evaluate the nitritation performance, continuous feeding tests were conducted using heat-shocked gel carriers treated at three temperatures. Stable nitritation was observed for 49 days when gel carriers were heat shocked at 60-90 °C for 1 h. However, because nitrate production, i.e., nitratation, was observed after 77 days, the gel carriers were heat shocked again. Consequently, nitratation stopped immediately and nitritation restarted after 14 days. These results clearly show that this technique is effective for suppressing nitratation.

    Original languageEnglish
    Pages (from-to)265-270
    Number of pages6
    JournalProcess Biochemistry
    Volume43
    Issue number3
    DOIs
    Publication statusPublished - 2008 Mar

    Fingerprint

    Bacteria
    Gels
    Hot Temperature
    Ammonium Compounds
    Shock
    Nitrites
    Sewage
    Temperature
    Sewage sludge
    Waste Water
    Nitrates
    Population
    Wastewater
    Costs and Cost Analysis
    Costs

    Keywords

    • Heat shock
    • Immobilization
    • Inhibition
    • Nitrification
    • Nitritation
    • Nitrite

    ASJC Scopus subject areas

    • Biochemistry
    • Organic Chemistry
    • Engineering (miscellaneous)
    • Industrial and Manufacturing Engineering

    Cite this

    Novel nitritation process using heat-shocked nitrifying bacteria entrapped in gel carriers. / Isaka, Kazuichi; Sumino, Tatsuo; Tsuneda, Satoshi.

    In: Process Biochemistry, Vol. 43, No. 3, 03.2008, p. 265-270.

    Research output: Contribution to journalArticle

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