Novel technique of overlaying a poly-L-lactic acid nanosheet for adhesion prophylaxis and fixation of intraperitoneal onlay polypropylene mesh in a rabbit model

Keiichi Fujino, Manabu Kinoshita, Akihiro Saitoh, Hidekazu Yano, Kahoko Nishikawa, Toshinori Fujie, Keiichi Iwaya, Minoru Kakihara, Shinji Takeoka, Daizoh Saitoh, Yuji Tanaka

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background One problem with polypropylene mesh (PPM) used to repair abdominal wall hernias is dense adhesions to the visceral surface. The authors developed the biocompatible poly-L-lactic acid (PLLA) nanosheet (thickness\100 nm), which has the unique ability to adhere tightly to tissues but not to opposing tissues. This study investigated the antiadhesive and fixative characteristics of the PLLA nanosheet after placement of intraperitoneal onlay PPM (IPOM) overlaid with a PLLA nanosheet on intact peritoneum. Methods The PLLA nanosheet was fabricated by the spin-coating method and peeling technique with polyvinyl alcohol (PVA) as a supporting film. Two 1.5-cm-square pieces of mesh were implanted on each peritoneal side of the midline incision. The mesh was fixed to the peritoneum with a suture and then overlaid with a 4-cm-square piece of Seprafilm or nanosheet. To examine the fixative property, mesh was overlaid with Seprafilm or nanosheet without a fixed suture. After 4 weeks, mesh adhesion, inflammatory reaction, fixation, and dislocation of mesh were evaluated. Results Nanosheet-overlaid meshes were flexible and fit over the peritoneum. Adhesion was observed in 10% of the nanosheet-overlaid meshes and in 50% of the Seprafilm- overlaid meshes. The adhesion tenacity grade was significantly lower with the nanosheet-overlaid meshes (0.1 ± 0.1) than with the Seprafilm-overlaid meshes (1.0 ± 0.4) (p = 0.029), and the percentage of the adhesion area also was lower with the nanosheet-overlaid meshes (1.0 ± 1.0% vs 8.5 ± 3.2%; p = 0.037). The mean inflammatory cell counts were lower with the nanosheet- overlaid meshes (p = 0.0023). Regarding the fixative property, 37.5% of the nanosheet-overlaid meshes were fixated on the peritoneum, but no Seprafilm-overlaid mesh was fixated. Conclusion Overlaying of a PLLA nanosheet was effective for adhesion prophylaxis of intraperitoneal mesh. It also may have a possible beneficial effect on fixation of mesh.

Original languageEnglish
Pages (from-to)3428-3436
Number of pages9
JournalSurgical Endoscopy and Other Interventional Techniques
Volume25
Issue number10
DOIs
Publication statusPublished - 2011 Oct

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Inlays
Polypropylenes
Peritoneum
Fixatives
Rabbits
Sutures
Abdominal Hernia
Polyvinyl Alcohol
Abdominal Wall
Cell Count
Seprafilm
poly(lactic acid)

Keywords

  • Adhesion
  • Fixation
  • PLLA nanosheet
  • Polymer
  • Polypropylene
  • Pore

ASJC Scopus subject areas

  • Surgery

Cite this

Novel technique of overlaying a poly-L-lactic acid nanosheet for adhesion prophylaxis and fixation of intraperitoneal onlay polypropylene mesh in a rabbit model. / Fujino, Keiichi; Kinoshita, Manabu; Saitoh, Akihiro; Yano, Hidekazu; Nishikawa, Kahoko; Fujie, Toshinori; Iwaya, Keiichi; Kakihara, Minoru; Takeoka, Shinji; Saitoh, Daizoh; Tanaka, Yuji.

In: Surgical Endoscopy and Other Interventional Techniques, Vol. 25, No. 10, 10.2011, p. 3428-3436.

Research output: Contribution to journalArticle

Fujino, Keiichi ; Kinoshita, Manabu ; Saitoh, Akihiro ; Yano, Hidekazu ; Nishikawa, Kahoko ; Fujie, Toshinori ; Iwaya, Keiichi ; Kakihara, Minoru ; Takeoka, Shinji ; Saitoh, Daizoh ; Tanaka, Yuji. / Novel technique of overlaying a poly-L-lactic acid nanosheet for adhesion prophylaxis and fixation of intraperitoneal onlay polypropylene mesh in a rabbit model. In: Surgical Endoscopy and Other Interventional Techniques. 2011 ; Vol. 25, No. 10. pp. 3428-3436.
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abstract = "Background One problem with polypropylene mesh (PPM) used to repair abdominal wall hernias is dense adhesions to the visceral surface. The authors developed the biocompatible poly-L-lactic acid (PLLA) nanosheet (thickness\100 nm), which has the unique ability to adhere tightly to tissues but not to opposing tissues. This study investigated the antiadhesive and fixative characteristics of the PLLA nanosheet after placement of intraperitoneal onlay PPM (IPOM) overlaid with a PLLA nanosheet on intact peritoneum. Methods The PLLA nanosheet was fabricated by the spin-coating method and peeling technique with polyvinyl alcohol (PVA) as a supporting film. Two 1.5-cm-square pieces of mesh were implanted on each peritoneal side of the midline incision. The mesh was fixed to the peritoneum with a suture and then overlaid with a 4-cm-square piece of Seprafilm or nanosheet. To examine the fixative property, mesh was overlaid with Seprafilm or nanosheet without a fixed suture. After 4 weeks, mesh adhesion, inflammatory reaction, fixation, and dislocation of mesh were evaluated. Results Nanosheet-overlaid meshes were flexible and fit over the peritoneum. Adhesion was observed in 10{\%} of the nanosheet-overlaid meshes and in 50{\%} of the Seprafilm- overlaid meshes. The adhesion tenacity grade was significantly lower with the nanosheet-overlaid meshes (0.1 ± 0.1) than with the Seprafilm-overlaid meshes (1.0 ± 0.4) (p = 0.029), and the percentage of the adhesion area also was lower with the nanosheet-overlaid meshes (1.0 ± 1.0{\%} vs 8.5 ± 3.2{\%}; p = 0.037). The mean inflammatory cell counts were lower with the nanosheet- overlaid meshes (p = 0.0023). Regarding the fixative property, 37.5{\%} of the nanosheet-overlaid meshes were fixated on the peritoneum, but no Seprafilm-overlaid mesh was fixated. Conclusion Overlaying of a PLLA nanosheet was effective for adhesion prophylaxis of intraperitoneal mesh. It also may have a possible beneficial effect on fixation of mesh.",
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author = "Keiichi Fujino and Manabu Kinoshita and Akihiro Saitoh and Hidekazu Yano and Kahoko Nishikawa and Toshinori Fujie and Keiichi Iwaya and Minoru Kakihara and Shinji Takeoka and Daizoh Saitoh and Yuji Tanaka",
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T1 - Novel technique of overlaying a poly-L-lactic acid nanosheet for adhesion prophylaxis and fixation of intraperitoneal onlay polypropylene mesh in a rabbit model

AU - Fujino, Keiichi

AU - Kinoshita, Manabu

AU - Saitoh, Akihiro

AU - Yano, Hidekazu

AU - Nishikawa, Kahoko

AU - Fujie, Toshinori

AU - Iwaya, Keiichi

AU - Kakihara, Minoru

AU - Takeoka, Shinji

AU - Saitoh, Daizoh

AU - Tanaka, Yuji

PY - 2011/10

Y1 - 2011/10

N2 - Background One problem with polypropylene mesh (PPM) used to repair abdominal wall hernias is dense adhesions to the visceral surface. The authors developed the biocompatible poly-L-lactic acid (PLLA) nanosheet (thickness\100 nm), which has the unique ability to adhere tightly to tissues but not to opposing tissues. This study investigated the antiadhesive and fixative characteristics of the PLLA nanosheet after placement of intraperitoneal onlay PPM (IPOM) overlaid with a PLLA nanosheet on intact peritoneum. Methods The PLLA nanosheet was fabricated by the spin-coating method and peeling technique with polyvinyl alcohol (PVA) as a supporting film. Two 1.5-cm-square pieces of mesh were implanted on each peritoneal side of the midline incision. The mesh was fixed to the peritoneum with a suture and then overlaid with a 4-cm-square piece of Seprafilm or nanosheet. To examine the fixative property, mesh was overlaid with Seprafilm or nanosheet without a fixed suture. After 4 weeks, mesh adhesion, inflammatory reaction, fixation, and dislocation of mesh were evaluated. Results Nanosheet-overlaid meshes were flexible and fit over the peritoneum. Adhesion was observed in 10% of the nanosheet-overlaid meshes and in 50% of the Seprafilm- overlaid meshes. The adhesion tenacity grade was significantly lower with the nanosheet-overlaid meshes (0.1 ± 0.1) than with the Seprafilm-overlaid meshes (1.0 ± 0.4) (p = 0.029), and the percentage of the adhesion area also was lower with the nanosheet-overlaid meshes (1.0 ± 1.0% vs 8.5 ± 3.2%; p = 0.037). The mean inflammatory cell counts were lower with the nanosheet- overlaid meshes (p = 0.0023). Regarding the fixative property, 37.5% of the nanosheet-overlaid meshes were fixated on the peritoneum, but no Seprafilm-overlaid mesh was fixated. Conclusion Overlaying of a PLLA nanosheet was effective for adhesion prophylaxis of intraperitoneal mesh. It also may have a possible beneficial effect on fixation of mesh.

AB - Background One problem with polypropylene mesh (PPM) used to repair abdominal wall hernias is dense adhesions to the visceral surface. The authors developed the biocompatible poly-L-lactic acid (PLLA) nanosheet (thickness\100 nm), which has the unique ability to adhere tightly to tissues but not to opposing tissues. This study investigated the antiadhesive and fixative characteristics of the PLLA nanosheet after placement of intraperitoneal onlay PPM (IPOM) overlaid with a PLLA nanosheet on intact peritoneum. Methods The PLLA nanosheet was fabricated by the spin-coating method and peeling technique with polyvinyl alcohol (PVA) as a supporting film. Two 1.5-cm-square pieces of mesh were implanted on each peritoneal side of the midline incision. The mesh was fixed to the peritoneum with a suture and then overlaid with a 4-cm-square piece of Seprafilm or nanosheet. To examine the fixative property, mesh was overlaid with Seprafilm or nanosheet without a fixed suture. After 4 weeks, mesh adhesion, inflammatory reaction, fixation, and dislocation of mesh were evaluated. Results Nanosheet-overlaid meshes were flexible and fit over the peritoneum. Adhesion was observed in 10% of the nanosheet-overlaid meshes and in 50% of the Seprafilm- overlaid meshes. The adhesion tenacity grade was significantly lower with the nanosheet-overlaid meshes (0.1 ± 0.1) than with the Seprafilm-overlaid meshes (1.0 ± 0.4) (p = 0.029), and the percentage of the adhesion area also was lower with the nanosheet-overlaid meshes (1.0 ± 1.0% vs 8.5 ± 3.2%; p = 0.037). The mean inflammatory cell counts were lower with the nanosheet- overlaid meshes (p = 0.0023). Regarding the fixative property, 37.5% of the nanosheet-overlaid meshes were fixated on the peritoneum, but no Seprafilm-overlaid mesh was fixated. Conclusion Overlaying of a PLLA nanosheet was effective for adhesion prophylaxis of intraperitoneal mesh. It also may have a possible beneficial effect on fixation of mesh.

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KW - Fixation

KW - PLLA nanosheet

KW - Polymer

KW - Polypropylene

KW - Pore

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