Nuclear and mitochondrial DNA analyses reveal four genetically separated breeding units of the swordfish

S. Chow, H. Takeyama

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

Variants in the calmodulin gene intron 4 (CaM) and the mtDNA D-loop region (D-loop) detected by RFLP and nucleotide sequence analyses were used to investigate the global stock structure of the swordfish Xiphias gladius. Two alleles (A and B) were observed at the CaM locus among 8 samples (Mediterranean, Tarifa, four Atlantic areas, Indian and Pacific oceans) comprising 567 individuals. Genotype distributions at this locus within samples were in accordance with Hardy-Weinberg equilibrium. Significant differences in allele frequencies were observed between Mediterranean-Northwest Atlantic samples (A=0.347-0.493) and those from tropical Atlantic and Indo-Pacific (A=0.81-1). In the D-loop region, Alu I and Rsa I digestions detected 4 (A-D) and 8 (A-H) genotypes, respectively. The frequency of type C for Alu I was lower in Indo-Pacific samples (0.2) than Atlantic and Mediterranean samples (0.365-0.643). Frequency of type C for Rsa I increased from the Pacific (0.398) to the Northwest Atlantic (0.698) and Tarifa (0.949) samples, and the Mediterranean sample was fixed for type C. These samples were classified into three groups (Mediterranean-Tarifa, Atlantic and Indo-Pacific) using a heterogeneity test of mtDNA genotype distributions. Combined analysis of the CaM and D-loop loci indicated that there are at least four breeding units: Mediterranean, northwestern Atlantic, tropical to South Atlantic, and Indo-Pacific. (C) 2000 The Fisheries Society of the British Isles.

Original languageEnglish
Pages (from-to)1087-1098
Number of pages12
JournalJournal of Fish Biology
Volume56
Issue number5
DOIs
Publication statusPublished - 2000 Sep 26
Externally publishedYes

Keywords

  • Breeding units
  • Intron
  • Swordfish
  • mtDNA

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science

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