Nuclear compartmentalization is abolished during fission yeast meiosis

Kunio Arai, Masamitsu Sato, Kayoko Tanaka, Masayuki Yamamoto

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

In eukaryotic cells, the nuclear envelope partitions the nucleus from the cytoplasm. The fission yeast Schizosaccharomyces pombe undergoes closed mitosis in which the nuclear envelope persists rather than being broken down, as in higher eukaryotic cells [1]. It is therefore assumed that nucleocytoplasmic transport continues during the cell cycle [2]. Here we show that nuclear transport is, in fact, abolished specifically during anaphase of the second meiotic nuclear division. During that time, both nucleoplasmic and cytoplasmic proteins disperse throughout the cell, reminiscent of the open mitosis of higher eukaryotes, but the architecture of the S. pombe nuclear envelope itself persists. This functional alteration of the nucleocytoplasmic barrier is likely induced by spore wall formation, because ectopic induction of sporulation signaling leads to premature dispersion of nucleoplasmic proteins. A photobleaching assay demonstrated that nuclear envelope permeability increases abruptly at the onset of anaphase of the second meiotic division. The permeability was not altered when sporulation was inhibited by blocking the trafficking of forespore-membrane vesicles from the endoplasmic reticulum to the Golgi. The evidence indicates that yeast gametogenesis produces vesicle transport-mediated forespore membranes by inducing nuclear envelope permeabilization.

Original languageEnglish
Pages (from-to)1913-1918
Number of pages6
JournalCurrent Biology
Volume20
Issue number21
DOIs
Publication statusPublished - 2010 Nov 9
Externally publishedYes

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Schizosaccharomyces
Schizosaccharomyces pombe
Nuclear Envelope
nuclear membrane
Meiosis
meiosis
Yeast
Membranes
Photobleaching
eukaryotic cells
Anaphase
Cell Nucleus Active Transport
anaphase
Eukaryotic Cells
Assays
Proteins
sporulation
Mitosis
mitosis
Cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Nuclear compartmentalization is abolished during fission yeast meiosis. / Arai, Kunio; Sato, Masamitsu; Tanaka, Kayoko; Yamamoto, Masayuki.

In: Current Biology, Vol. 20, No. 21, 09.11.2010, p. 1913-1918.

Research output: Contribution to journalArticle

Arai, Kunio ; Sato, Masamitsu ; Tanaka, Kayoko ; Yamamoto, Masayuki. / Nuclear compartmentalization is abolished during fission yeast meiosis. In: Current Biology. 2010 ; Vol. 20, No. 21. pp. 1913-1918.
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