Nuclear localization of reporter genes activated by curved DNA

Koji Udagawa, Hajime Kimura, Hideyuki Tanabe, Takashi Ohyama

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Curved DNA structures with a left-handed superhelical conformation can activate eukaryotic transcription. Mechanistically, these structures favor binding to histone cores and can function as a docking site for sliding nucleosomes. Thus, promoters with this kind of curved DNA can adopt a more open structure, facilitating transcription initiation. However, whether the curved DNA segment can affect localization of a reporter gene is an open question. Localization of a gene in the nucleus often plays an important role in its expression and this phenomenon may also have a curved DNA-dependent mechanism. We examined this issue in transient and stable assay systems using a 180-bp synthetic curved DNA with a left-handed superhelical conformation. The results clearly showed that curved DNA of this kind does not have a property to deliver reporter constructs to nuclear positions that are preferable for transcription. We also identify the spatial location to which electroporation delivers a reporter plasmid in the nucleus.

Original languageEnglish
Pages (from-to)431-437
Number of pages7
JournalJournal of Bioscience and Bioengineering
Volume113
Issue number4
DOIs
Publication statusPublished - 2012 Apr 1

Keywords

  • Cell nucleus
  • Chromatin engineering
  • Curved DNA
  • Electroporation
  • Gene expression
  • Gene localization
  • Transgene

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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