Nuclear localization of reporter genes activated by curved DNA

Koji Udagawa, Hajime Kimura, Hideyuki Tanabe, Takashi Ohyama

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Curved DNA structures with a left-handed superhelical conformation can activate eukaryotic transcription. Mechanistically, these structures favor binding to histone cores and can function as a docking site for sliding nucleosomes. Thus, promoters with this kind of curved DNA can adopt a more open structure, facilitating transcription initiation. However, whether the curved DNA segment can affect localization of a reporter gene is an open question. Localization of a gene in the nucleus often plays an important role in its expression and this phenomenon may also have a curved DNA-dependent mechanism. We examined this issue in transient and stable assay systems using a 180-bp synthetic curved DNA with a left-handed superhelical conformation. The results clearly showed that curved DNA of this kind does not have a property to deliver reporter constructs to nuclear positions that are preferable for transcription. We also identify the spatial location to which electroporation delivers a reporter plasmid in the nucleus.

    Original languageEnglish
    Pages (from-to)431-437
    Number of pages7
    JournalJournal of Bioscience and Bioengineering
    Volume113
    Issue number4
    DOIs
    Publication statusPublished - 2012 Apr

    Fingerprint

    Reporter Genes
    DNA
    Genes
    Transcription
    Conformations
    Electroporation
    Nucleosomes
    Histones
    Assays
    Plasmids

    Keywords

    • Cell nucleus
    • Chromatin engineering
    • Curved DNA
    • Electroporation
    • Gene expression
    • Gene localization
    • Transgene

    ASJC Scopus subject areas

    • Biotechnology
    • Applied Microbiology and Biotechnology
    • Bioengineering

    Cite this

    Nuclear localization of reporter genes activated by curved DNA. / Udagawa, Koji; Kimura, Hajime; Tanabe, Hideyuki; Ohyama, Takashi.

    In: Journal of Bioscience and Bioengineering, Vol. 113, No. 4, 04.2012, p. 431-437.

    Research output: Contribution to journalArticle

    Udagawa, Koji ; Kimura, Hajime ; Tanabe, Hideyuki ; Ohyama, Takashi. / Nuclear localization of reporter genes activated by curved DNA. In: Journal of Bioscience and Bioengineering. 2012 ; Vol. 113, No. 4. pp. 431-437.
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