Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint

Valentina Rossio, Takeshi Michimoto, Takeshi Sasaki, Iwai Ohbayashi, Yoshiko Kikuchi, Satoshi Yoshida

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Cdc55, a regulatory B-subunit of protein phosphatase 2A (PP2A) complex, is essential for the spindle assembly checkpoint (SAC) in budding yeast, but the regulation and molecular targets of PP2A-Cdc55 have not been clearly defined or are controversial. Here, we show that an important target of Cdc55 in the SAC is the anaphase-promoting complex (APC) coupled with Cdc20 and that APC-Cdc20 is kept inactive by dephosphorylation by nuclear PP2A-Cdc55 when spindle is damaged. By isolating a new class of Cdc55 mutants specifically defective in the SAC and by artificially manipulating nucleocytoplasmic distribution of Cdc55, we further show that nuclear Cdc55 is essential for the SAC. Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by Zds1 family proteins is important for tight control of SAC and mitotic progression.

Original languageEnglish
Pages (from-to)4396-4405
Number of pages10
JournalJournal of Cell Science
Volume126
Issue number19
DOIs
Publication statusPublished - 2013 Oct 18
Externally publishedYes

Fingerprint

Anaphase-Promoting Complex-Cyclosome
M Phase Cell Cycle Checkpoints
Protein Phosphatase 2
Phosphoprotein Phosphatases
Saccharomycetales
Carrier Proteins

Keywords

  • Anaphase-promoting complex
  • APC
  • Cdc55
  • PP2A
  • Protein phosphatase 2A
  • SAC
  • Spindle assembly checkpoint
  • Zds1

ASJC Scopus subject areas

  • Cell Biology

Cite this

Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint. / Rossio, Valentina; Michimoto, Takeshi; Sasaki, Takeshi; Ohbayashi, Iwai; Kikuchi, Yoshiko; Yoshida, Satoshi.

In: Journal of Cell Science, Vol. 126, No. 19, 18.10.2013, p. 4396-4405.

Research output: Contribution to journalArticle

Rossio, V, Michimoto, T, Sasaki, T, Ohbayashi, I, Kikuchi, Y & Yoshida, S 2013, 'Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint', Journal of Cell Science, vol. 126, no. 19, pp. 4396-4405. https://doi.org/10.1242/jcs.127365
Rossio, Valentina ; Michimoto, Takeshi ; Sasaki, Takeshi ; Ohbayashi, Iwai ; Kikuchi, Yoshiko ; Yoshida, Satoshi. / Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint. In: Journal of Cell Science. 2013 ; Vol. 126, No. 19. pp. 4396-4405.
@article{65da1efc1a2349d4aac156975ec75f8b,
title = "Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint",
abstract = "Cdc55, a regulatory B-subunit of protein phosphatase 2A (PP2A) complex, is essential for the spindle assembly checkpoint (SAC) in budding yeast, but the regulation and molecular targets of PP2A-Cdc55 have not been clearly defined or are controversial. Here, we show that an important target of Cdc55 in the SAC is the anaphase-promoting complex (APC) coupled with Cdc20 and that APC-Cdc20 is kept inactive by dephosphorylation by nuclear PP2A-Cdc55 when spindle is damaged. By isolating a new class of Cdc55 mutants specifically defective in the SAC and by artificially manipulating nucleocytoplasmic distribution of Cdc55, we further show that nuclear Cdc55 is essential for the SAC. Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by Zds1 family proteins is important for tight control of SAC and mitotic progression.",
keywords = "Anaphase-promoting complex, APC, Cdc55, PP2A, Protein phosphatase 2A, SAC, Spindle assembly checkpoint, Zds1",
author = "Valentina Rossio and Takeshi Michimoto and Takeshi Sasaki and Iwai Ohbayashi and Yoshiko Kikuchi and Satoshi Yoshida",
year = "2013",
month = "10",
day = "18",
doi = "10.1242/jcs.127365",
language = "English",
volume = "126",
pages = "4396--4405",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "Company of Biologists Ltd",
number = "19",

}

TY - JOUR

T1 - Nuclear PP2A-Cdc55 prevents APC-Cdc20 activation during the spindle assembly checkpoint

AU - Rossio, Valentina

AU - Michimoto, Takeshi

AU - Sasaki, Takeshi

AU - Ohbayashi, Iwai

AU - Kikuchi, Yoshiko

AU - Yoshida, Satoshi

PY - 2013/10/18

Y1 - 2013/10/18

N2 - Cdc55, a regulatory B-subunit of protein phosphatase 2A (PP2A) complex, is essential for the spindle assembly checkpoint (SAC) in budding yeast, but the regulation and molecular targets of PP2A-Cdc55 have not been clearly defined or are controversial. Here, we show that an important target of Cdc55 in the SAC is the anaphase-promoting complex (APC) coupled with Cdc20 and that APC-Cdc20 is kept inactive by dephosphorylation by nuclear PP2A-Cdc55 when spindle is damaged. By isolating a new class of Cdc55 mutants specifically defective in the SAC and by artificially manipulating nucleocytoplasmic distribution of Cdc55, we further show that nuclear Cdc55 is essential for the SAC. Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by Zds1 family proteins is important for tight control of SAC and mitotic progression.

AB - Cdc55, a regulatory B-subunit of protein phosphatase 2A (PP2A) complex, is essential for the spindle assembly checkpoint (SAC) in budding yeast, but the regulation and molecular targets of PP2A-Cdc55 have not been clearly defined or are controversial. Here, we show that an important target of Cdc55 in the SAC is the anaphase-promoting complex (APC) coupled with Cdc20 and that APC-Cdc20 is kept inactive by dephosphorylation by nuclear PP2A-Cdc55 when spindle is damaged. By isolating a new class of Cdc55 mutants specifically defective in the SAC and by artificially manipulating nucleocytoplasmic distribution of Cdc55, we further show that nuclear Cdc55 is essential for the SAC. Because the Cdc55-binding proteins Zds1 and Zds2 inhibit both nuclear accumulation of Cdc55 and SAC activity, we propose that spatial control of PP2A by Zds1 family proteins is important for tight control of SAC and mitotic progression.

KW - Anaphase-promoting complex

KW - APC

KW - Cdc55

KW - PP2A

KW - Protein phosphatase 2A

KW - SAC

KW - Spindle assembly checkpoint

KW - Zds1

UR - http://www.scopus.com/inward/record.url?scp=84885435652&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84885435652&partnerID=8YFLogxK

U2 - 10.1242/jcs.127365

DO - 10.1242/jcs.127365

M3 - Article

C2 - 23886942

AN - SCOPUS:84885435652

VL - 126

SP - 4396

EP - 4405

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 19

ER -