Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene

Rippei Hayashi; Takuya Ueda; Mary A. Farwell; Nono Takeuchi

doi:10.1016/j.mito.2006.10.005

Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene

Rippei Hayashi, Takuya Ueda, Mary A. Farwell, Nono Takeuchi

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2α/β binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2α/β-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.

Original language	English
Pages (from-to)	195-203
Number of pages	9
Journal	Mitochondrion
Volume	7
Issue number	3
DOIs	https://doi.org/10.1016/j.mito.2006.10.005
Publication status	Published - 2007 May 1
Externally published	Yes

Keywords

Initiation factor 2
Mitochondria
Nuclear respiratory factor 2
Transcriptional regulation
Translation

ASJC Scopus subject areas

Molecular Medicine
Molecular Biology
Cell Biology

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Cite this

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title = "Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene",

abstract = "We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2α/β binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2α/β-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.",

keywords = "Initiation factor 2, Mitochondria, Nuclear respiratory factor 2, Transcriptional regulation, Translation",

author = "Rippei Hayashi and Takuya Ueda and Farwell, {Mary A.} and Nono Takeuchi",

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T1 - Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene

AU - Hayashi, Rippei

AU - Ueda, Takuya

AU - Farwell, Mary A.

AU - Takeuchi, Nono

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Y1 - 2007/5/1

N2 - We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2α/β binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2α/β-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.

AB - We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2α/β binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2α/β-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.

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KW - Nuclear respiratory factor 2

KW - Transcriptional regulation

KW - Translation

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