Abstract
We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2α/β binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2α/β-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.
Original language | English |
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Pages (from-to) | 195-203 |
Number of pages | 9 |
Journal | Mitochondrion |
Volume | 7 |
Issue number | 3 |
DOIs | |
Publication status | Published - 2007 May |
Externally published | Yes |
Keywords
- Initiation factor 2
- Mitochondria
- Nuclear respiratory factor 2
- Transcriptional regulation
- Translation
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Cell Biology