Nuclear respiratory factor 2 activates transcription of human mitochondrial translation initiation factor 2 gene

Rippei Hayashi, Takuya Ueda, Mary A. Farwell, Nono Takeuchi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We studied the transcriptional regulation of the human mitochondrial translation initiation factor 2 (IF2mt) gene. The minimal promoter region for the human IF2mt gene contains binding sites for Nuclear Respiratory Factor 2 (NRF-2), which is often involved in the transcription of mitochondrial-related genes. Electrophoresis mobility shift assay (EMSA) analyses indicated that NRF-2α/β binds to the IF2mt promoter. Reporter assays, where HEK293T cells were co-transfected with an NRF-2α/β-expressing vector and/or an IF2mt promoter reporter vector, revealed that NRF-2 trans-activates the IF2mt promoter. NRF-2 sites were also found in the promoters of several other mitochondrial translation factors, which suggests NRF-2 may play a key role in the regulation of mitochondrial protein synthesis.

Original languageEnglish
Pages (from-to)195-203
Number of pages9
JournalMitochondrion
Volume7
Issue number3
DOIs
Publication statusPublished - 2007 May 1

Keywords

  • Initiation factor 2
  • Mitochondria
  • Nuclear respiratory factor 2
  • Transcriptional regulation
  • Translation

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology
  • Cell Biology

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