Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast

Masamitsu Sato, Naoyuki Okada, Yasutaka Kakui, Masayuki Yamamoto, Minoru Yoshida, Takashi Toda

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Ran GTPase activates several target molecules to induce microtubule formation around the chromosomes and centrosomes. In fission yeast, in which the nuclear envelope does not break down during mitosis, Ran targets the centrosomal transforming acidic coiled-coil (TACC) protein Alp7 for spindle formation. Alp7 accumulates in the nucleus only during mitosis, although its underlying mechanism remains elusive. Here, we investigate the behaviour of Alp7 and its binding partner, Alp14/TOG, throughout the cell cycle. Interestingly, Alp7 enters the nucleus during interphase but is subsequently exported to the cytoplasm by the Exportin-dependent nuclear export machinery. The continuous nuclear export of Alp7 during interphase is essential for maintaining the array-like cytoplasmic microtubule structure. The mitosis-specific nuclear accumulation of Alp7 seems to be under the control of cyclin-dependent kinase (CDK). These results indicate that the spatiotemporal regulation of microtubule formation is established by the Alp7/TACC-Alp14/TOG complex through the coordinated interplay of Ran and CDK.

Original languageEnglish
Pages (from-to)1161-1167
Number of pages7
JournalEMBO Reports
Volume10
Issue number10
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Cell Nucleus Active Transport
Cyclin-Dependent Kinases
Schizosaccharomyces
Mitosis
Microtubules
Yeast
Interphase
Karyopherins
GTP Phosphohydrolases
Chromosomes
Cytoplasmic Structures
Machinery
Centrosome
Nuclear Envelope
Cells
Molecules
Cell Cycle
Cytoplasm
Proteins

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry

Cite this

Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast. / Sato, Masamitsu; Okada, Naoyuki; Kakui, Yasutaka; Yamamoto, Masayuki; Yoshida, Minoru; Toda, Takashi.

In: EMBO Reports, Vol. 10, No. 10, 2009, p. 1161-1167.

Research output: Contribution to journalArticle

Sato, Masamitsu ; Okada, Naoyuki ; Kakui, Yasutaka ; Yamamoto, Masayuki ; Yoshida, Minoru ; Toda, Takashi. / Nucleocytoplasmic transport of Alp7/TACC organizes spatiotemporal microtubule formation in fission yeast. In: EMBO Reports. 2009 ; Vol. 10, No. 10. pp. 1161-1167.
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