TY - JOUR
T1 - Nucleosome destabilization by nuclear non-coding RNAs
AU - Fujita, Risa
AU - Yamamoto, Tatsuro
AU - Arimura, Yasuhiro
AU - Fujiwara, Saori
AU - Tachiwana, Hiroaki
AU - Ichikawa, Yuichi
AU - Sakata, Yuka
AU - Yang, Liying
AU - Maruyama, Reo
AU - Hamada, Michiaki
AU - Nakao, Mitsuyoshi
AU - Saitoh, Noriko
AU - Kurumizaka, Hitoshi
N1 - Funding Information:
We are grateful to Ms. Yukari Iikura (The University of Tokyo) for her assistance. This work was supported in part by JSPS KAKENHI grant numbers JP17H01408 [to H.K.], JP18H05534 [to H.K.], JP18H05531 [to N.S.], JP16H04744 [to N.S.], JP18K19479 [to M. N.], JP17K15043 [to Y.A.], JP19K23714 [to R.F.], JP19K23736 [to T.Y.], and JP19K21184 [to Y.I.]. This work was partly supported by JST CREST grant number JPMJCR16G1 [to H.K.], JST ERATO grant number JPMJER1901 [to H.K.], and also by the Platform Project for Supporting Drug Discovery and Life Science Research (Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS)) from AMED under Grant Number JP19am0101076 [to H.K.], and by grants from the Takeda Science Foundation [to N.S] and AMED CREST grant number JP16gm0510007 [to M.N.], and Mitsubishi Foundation [to M.N.]. R.F. was supported by a JSPS Research Fellowship for Young Scientists, JP16J10043. Funding for open access charge: The University of Tokyo.
Publisher Copyright:
© 2020, The Author(s).
PY - 2020/12/1
Y1 - 2020/12/1
N2 - In the nucleus, genomic DNA is wrapped around histone octamers to form nucleosomes. In principle, nucleosomes are substantial barriers to transcriptional activities. Nuclear non-coding RNAs (ncRNAs) are proposed to function in chromatin conformation modulation and transcriptional regulation. However, it remains unclear how ncRNAs affect the nucleosome structure. Eleanors are clusters of ncRNAs that accumulate around the estrogen receptor-α (ESR1) gene locus in long-term estrogen deprivation (LTED) breast cancer cells, and markedly enhance the transcription of the ESR1 gene. Here we detected nucleosome depletion around the transcription site of Eleanor2, the most highly expressed Eleanor in the LTED cells. We found that the purified Eleanor2 RNA fragment drastically destabilized the nucleosome in vitro. This activity was also exerted by other ncRNAs, but not by poly(U) RNA or DNA. The RNA-mediated nucleosome destabilization may be a common feature among natural nuclear RNAs, and may function in transcription regulation in chromatin.
AB - In the nucleus, genomic DNA is wrapped around histone octamers to form nucleosomes. In principle, nucleosomes are substantial barriers to transcriptional activities. Nuclear non-coding RNAs (ncRNAs) are proposed to function in chromatin conformation modulation and transcriptional regulation. However, it remains unclear how ncRNAs affect the nucleosome structure. Eleanors are clusters of ncRNAs that accumulate around the estrogen receptor-α (ESR1) gene locus in long-term estrogen deprivation (LTED) breast cancer cells, and markedly enhance the transcription of the ESR1 gene. Here we detected nucleosome depletion around the transcription site of Eleanor2, the most highly expressed Eleanor in the LTED cells. We found that the purified Eleanor2 RNA fragment drastically destabilized the nucleosome in vitro. This activity was also exerted by other ncRNAs, but not by poly(U) RNA or DNA. The RNA-mediated nucleosome destabilization may be a common feature among natural nuclear RNAs, and may function in transcription regulation in chromatin.
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U2 - 10.1038/s42003-020-0784-9
DO - 10.1038/s42003-020-0784-9
M3 - Article
C2 - 32047236
AN - SCOPUS:85079338947
SN - 2399-3642
VL - 3
JO - Communications Biology
JF - Communications Biology
IS - 1
M1 - 60
ER -
