Nucleosome formation activity of human somatic Nuclear Autoantigenic Sperm Protein (sNASP)

Akihisa Osakabe, Hiroaki Tachiwana, Takaaki Matsunaga, Tatsuya Shiga, Ryu Suke Nozawa, Chikashi Obuse, Hitoshi Kurumizaka

    Research output: Contribution to journalArticle

    44 Citations (Scopus)

    Abstract

    NASP (nuclear autoantigenic sperm protein) is a member of the N1/N2 family, which is widely conserved among eukaryotes. Human NASP reportedly prefers to bind to histones H3·H4 and the linker histone H1, as compared with H2A·H2B, and is anticipated to function as an H3·H4 chaperone for nucleosome assembly. However, the direct nucleosome assembly activity of human NASP has not been reported so far. In humans, two spliced isoforms, somatic and testicular NASPs (sNASP and tNASP, respectively) were identified. In the present study we purified human sNASP and found that sNASP efficiently promoted the assembly of nucleosomes containing the conventional H3.1, H3.2, H3.3, or centromere-specific CENP-A. On the other hand, sNASP inefficiently promoted nucleosome assembly with H3T, a testis-specific H3 variant. Mutational analyses revealed that the Met-71 residue of H3T is responsible for this inefficient nucleosome formation by sNASP. Tetrasomes, composed of the H3·H4 tetramer and DNA without H2A·H2B, were efficiently formed by the sNASP-mediated nucleosome-assembly reaction. A deletion analysis of sNASP revealed that the central region, amino acid residues 26-325, of sNASP is responsible for nucleosome assembly in vitro. These experiments are the first demonstration that human NASP directly promotes nucleosome assembly and provide compelling evidence that sNASP is a bona fide histone chaperone for H3·H4.

    Original languageEnglish
    Pages (from-to)11913-11921
    Number of pages9
    JournalJournal of Biological Chemistry
    Volume285
    Issue number16
    DOIs
    Publication statusPublished - 2010 Apr 16

    Fingerprint

    Nucleosomes
    Human Activities
    Spermatozoa
    Proteins
    Histones
    Histone Chaperones
    Centromere
    Eukaryota
    Testis
    Protein Isoforms
    Demonstrations

    ASJC Scopus subject areas

    • Biochemistry
    • Cell Biology
    • Molecular Biology

    Cite this

    Osakabe, A., Tachiwana, H., Matsunaga, T., Shiga, T., Nozawa, R. S., Obuse, C., & Kurumizaka, H. (2010). Nucleosome formation activity of human somatic Nuclear Autoantigenic Sperm Protein (sNASP). Journal of Biological Chemistry, 285(16), 11913-11921. https://doi.org/10.1074/jbc.M109.083238

    Nucleosome formation activity of human somatic Nuclear Autoantigenic Sperm Protein (sNASP). / Osakabe, Akihisa; Tachiwana, Hiroaki; Matsunaga, Takaaki; Shiga, Tatsuya; Nozawa, Ryu Suke; Obuse, Chikashi; Kurumizaka, Hitoshi.

    In: Journal of Biological Chemistry, Vol. 285, No. 16, 16.04.2010, p. 11913-11921.

    Research output: Contribution to journalArticle

    Osakabe, A, Tachiwana, H, Matsunaga, T, Shiga, T, Nozawa, RS, Obuse, C & Kurumizaka, H 2010, 'Nucleosome formation activity of human somatic Nuclear Autoantigenic Sperm Protein (sNASP)', Journal of Biological Chemistry, vol. 285, no. 16, pp. 11913-11921. https://doi.org/10.1074/jbc.M109.083238
    Osakabe A, Tachiwana H, Matsunaga T, Shiga T, Nozawa RS, Obuse C et al. Nucleosome formation activity of human somatic Nuclear Autoantigenic Sperm Protein (sNASP). Journal of Biological Chemistry. 2010 Apr 16;285(16):11913-11921. https://doi.org/10.1074/jbc.M109.083238
    Osakabe, Akihisa ; Tachiwana, Hiroaki ; Matsunaga, Takaaki ; Shiga, Tatsuya ; Nozawa, Ryu Suke ; Obuse, Chikashi ; Kurumizaka, Hitoshi. / Nucleosome formation activity of human somatic Nuclear Autoantigenic Sperm Protein (sNASP). In: Journal of Biological Chemistry. 2010 ; Vol. 285, No. 16. pp. 11913-11921.
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