Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria

Taeko Nishiwaki, Hideo Iwasaki, Masahiro Ishiura, Takao Kondo

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

A negative feedback control of kaiC expression by KaiC protein has been proposed to generate a basic oscillation of the circadian clock in the cyanobacterium Synechococcus sp. PCC 7942. KaiC has two P loops or Walker's motif As, that are potential ATP-/GTP-binding motifs and DXXG motifs conserved in various GTP-binding proteins. Herein, we demonstrate that in vitro KaiC binds ATP and, with lower affinity, GTP. Point mutation by site- directed mutagenesis of P loop 1 completely nullified the circadian rhythm of kaiBC expression and markedly reduced ATP-binding activity. Moreover, KaiC can be autophosphorylated in vitro. These results suggest that the nucleotide-binding activity of KaiC plays important roles in the generation of circadian oscillation in cyanobacteria.

Original languageEnglish
Pages (from-to)495-499
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number1
DOIs
Publication statusPublished - 2000 Jan 4
Externally publishedYes

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Cyanobacteria
Nucleotides
Adenosine Triphosphate
Guanosine Triphosphate
Synechococcus
Circadian Clocks
Proteins
Circadian Rhythm
Site-Directed Mutagenesis
GTP-Binding Proteins
Point Mutation
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria. / Nishiwaki, Taeko; Iwasaki, Hideo; Ishiura, Masahiro; Kondo, Takao.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, No. 1, 04.01.2000, p. 495-499.

Research output: Contribution to journalArticle

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