Nucleotide sequence and expression of relaxin-like gonad-stimulating peptide gene in starfish Asterina pectinifera

Shogo Haraguchi, Narumi Ikeda, Michiko Abe, Kazuyoshi Tsutsui, Masatoshi Mita

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    Starfish gonad-stimulating substance (GSS) is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to gonadotropins in vertebrates. Because GSS belongs to the relaxin-like peptide family, we propose renaming for starfish gonadotropic hormone as relaxin-like gonad-stimulating peptide (RGP). This study examined the primary structure and expression regulation of the RGP gene in starfish Asterina pectinifera. RGP consisted of 3896 base pairs (bp) divided over two exons, exon 1 of 208 bp and exon 2 of 2277 bp, and one intron of 1411 bp. Promoter sequences, CAAT and TATA boxes, were present in the 5'-upstream region of the coding DNA sequence of RGP. The transcript was 2485 bases (b) in length. The AAUAAA polyadenylation signal was found in 3'-untranslated region over 2 kb away from the stop codon. This showed that only 14% of the RGP mRNA was translated into the peptide, because a size of the open-reading frame was 351 b. Furthermore, an analysis by using real-time quantitative PCR with specific primers for RGP showed that mRNA of RGP was expressed at high levels in the radial nerves. Expression was also observed in the cardiac stomachs, although the level was low, and trace levels were detected in the gonads, pyloric caeca and tube feet. This result suggests that the RGP gene is transcribed mainly in the radial nerves of A. pectinifera.

    Original languageEnglish
    Pages (from-to)115-119
    Number of pages5
    JournalGeneral and Comparative Endocrinology
    Volume227
    DOIs
    Publication statusPublished - 2016 Feb 1

    Fingerprint

    Asterina
    Asterina pectinifera
    relaxin
    Starfish
    Relaxin
    Asteroidea
    Gonads
    gonads
    peptides
    nucleotide sequences
    Peptides
    Genes
    genes
    Base Pairing
    exons
    Radial Nerve
    Exons
    Invertebrate Hormones
    nerve tissue
    TATA box

    Keywords

    • Coding-DNA sequence
    • Gene
    • Relaxin-like gonad-stimulating peptide (RGP)
    • Starfish
    • Transcription

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Nucleotide sequence and expression of relaxin-like gonad-stimulating peptide gene in starfish Asterina pectinifera. / Haraguchi, Shogo; Ikeda, Narumi; Abe, Michiko; Tsutsui, Kazuyoshi; Mita, Masatoshi.

    In: General and Comparative Endocrinology, Vol. 227, 01.02.2016, p. 115-119.

    Research output: Contribution to journalArticle

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