Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Tadashi Matsunaga; Hideaki Mihashita; Masato Miyake; J. Grant Burgess

doi:10.1016/0014-5793(91)80603-Z

Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Tadashi Matsunaga^*, Hideaki Mihashita, Masato Miyake, J. Grant Burgess

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

Original language	English
Pages (from-to)	263-266
Number of pages	4
Journal	FEBS Letters
Volume	283
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)80603-Z
Publication status	Published - 1991 Jun 3
Externally published	Yes

Keywords

Marine Rhodobacter
Plasmid
Replication region
Sequence

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology
Cell Biology
Genetics
Structural Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/0014-5793(91)80603-Z

Cite this

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title = "Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31",

abstract = "The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.",

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day = "3",

doi = "10.1016/0014-5793(91)80603-Z",

language = "English",

volume = "283",

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TY - JOUR

T1 - Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

AU - Matsunaga, Tadashi

AU - Mihashita, Hideaki

AU - Miyake, Masato

AU - Burgess, J. Grant

PY - 1991/6/3

Y1 - 1991/6/3

N2 - The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

AB - The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

KW - Marine Rhodobacter

KW - Plasmid

KW - Replication region

KW - Sequence

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DO - 10.1016/0014-5793(91)80603-Z

M3 - Article

C2 - 2044764

AN - SCOPUS:0025801731

VL - 283

SP - 263

EP - 266

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -

Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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