Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Tadashi Matsunaga; Hideaki Mihashita; Masato Miyake; J. Grant Burgess

doi:10.1016/0014-5793(91)80603-Z

Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Tadashi Matsunaga, Hideaki Mihashita, Masato Miyake, J. Grant Burgess

Research output: Contribution to journal › Article

5 Citations (Scopus)

Abstract

The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

Original language	English
Pages (from-to)	263-266
Number of pages	4
Journal	FEBS Letters
Volume	283
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)80603-Z
Publication status	Published - 1991 Jun 3
Externally published	Yes

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Keywords

Marine Rhodobacter
Plasmid
Replication region
Sequence

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology
Cell Biology
Genetics
Structural Biology

Cite this

Matsunaga, T., Mihashita, H., Miyake, M., & Burgess, J. G. (1991). Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31. FEBS Letters, 283(2), 263-266. https://doi.org/10.1016/0014-5793(91)80603-Z

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Access to Document

10.1016/0014-5793(91)80603-Z