Abstract
The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.
Original language | English |
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Pages (from-to) | 263-266 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 283 |
Issue number | 2 |
DOIs | |
Publication status | Published - 1991 Jun 3 |
Externally published | Yes |
Keywords
- Marine Rhodobacter
- Plasmid
- Replication region
- Sequence
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
- Cell Biology
- Genetics
- Structural Biology