Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Tadashi Matsunaga; Hideaki Mihashita; Masato Miyake; J. Grant Burgess

doi:10.1016/0014-5793(91)80603-Z

Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Tadashi Matsunaga^*, Hideaki Mihashita, Masato Miyake, J. Grant Burgess

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

Original language	English
Pages (from-to)	263-266
Number of pages	4
Journal	FEBS Letters
Volume	283
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(91)80603-Z
Publication status	Published - 1991 Jun 3
Externally published	Yes

Keywords

Marine Rhodobacter
Plasmid
Replication region
Sequence

ASJC Scopus subject areas

Biochemistry
Biophysics
Molecular Biology
Cell Biology
Genetics
Structural Biology

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10.1016/0014-5793(91)80603-Z

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abstract = "The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.",

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year = "1991",

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doi = "10.1016/0014-5793(91)80603-Z",

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T1 - Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

AU - Matsunaga, Tadashi

AU - Mihashita, Hideaki

AU - Miyake, Masato

AU - Burgess, J. Grant

PY - 1991/6/3

Y1 - 1991/6/3

N2 - The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

AB - The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

KW - Marine Rhodobacter

KW - Plasmid

KW - Replication region

KW - Sequence

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DO - 10.1016/0014-5793(91)80603-Z

M3 - Article

C2 - 2044764

AN - SCOPUS:0025801731

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SP - 263

EP - 266

JO - FEBS Letters

JF - FEBS Letters

SN - 0014-5793

IS - 2

ER -

Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Abstract

Keywords

ASJC Scopus subject areas

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