Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Tadashi Matsunaga, Hideaki Mihashita, Masato Miyake, J. Grant Burgess

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

Original languageEnglish
Pages (from-to)263-266
Number of pages4
JournalFEBS Letters
Volume283
Issue number2
DOIs
Publication statusPublished - 1991 Jun 3
Externally publishedYes

Fingerprint

Rhodobacter
Plasmids
Nucleotides
Bacteriophage P1
Bacteriophages
Replication Origin
Base Pairing
Bacteria
Mutation

Keywords

  • Marine Rhodobacter
  • Plasmid
  • Replication region
  • Sequence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

Cite this

Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31. / Matsunaga, Tadashi; Mihashita, Hideaki; Miyake, Masato; Burgess, J. Grant.

In: FEBS Letters, Vol. 283, No. 2, 03.06.1991, p. 263-266.

Research output: Contribution to journalArticle

Matsunaga, T, Mihashita, H, Miyake, M & Burgess, JG 1991, 'Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31', FEBS Letters, vol. 283, no. 2, pp. 263-266. https://doi.org/10.1016/0014-5793(91)80603-Z
Matsunaga, Tadashi ; Mihashita, Hideaki ; Miyake, Masato ; Burgess, J. Grant. / Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31. In: FEBS Letters. 1991 ; Vol. 283, No. 2. pp. 263-266.
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