Nucleotide sequence of the replication region of the marine Rhodobacter plasmid pRD31

Tadashi Matsunaga, Hideaki Mihashita, Masato Miyake, J. Grant Burgess

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The minimum region required for the replication of the marine Rhodabacter endogenous plasmid pRD31 has been sequenced. This region is located on a 1367-bp HincII-PstI restriction fragment and is 62% rich in G-C base pairs. A region homologous to the bacteriophage P1 repA promoter, which overlaps two inverted repeats, has been identified. Plasmids with mutations in this 1367-bp region could not replicate in marine Rhodobacter hosts. This is the first identified replication origin of a photosynthetic bacterium.

Original languageEnglish
Pages (from-to)263-266
Number of pages4
JournalFEBS Letters
Issue number2
Publication statusPublished - 1991 Jun 3
Externally publishedYes



  • Marine Rhodobacter
  • Plasmid
  • Replication region
  • Sequence

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology
  • Genetics
  • Structural Biology

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