Occurrence and determination of a natriuretic hormone, 2,7,8-trimethyl- 2-(β-carboxyethyl)-6-hydroxy chroman, in rat plasma, urine, and bile

Akihiro Hattori, Takeshi Fukushima, Kazuhiro Imai

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The occurrence of a new natriuretic hormone, 2,7,8-trimethyl-2-(β- carboxyethyl)-6-hydroxy chroman (LLU-α, γ-CEHC) in rat plasma was demonstrated and its concentration was determined using a coupled-column HPLC with a fluorometric derivatization with 4-N,N-dimethylaminosulfonyl-7- piperazino-2,1,3-benzoxadiazole (DBD-PZ) followed by O-acetylation. The concentration of LLU-α was 328 ± 113 nM in rat plasma (N = 5). LLU-α was found in not only urine, but also bile, suggesting an enterohepatic circulation in body. We also assigned the configuration at C-2 of LLU-α in these biological fluids as (S)-form by an HPLC with a chiral column. The LLU- α concentration decreased significantly by fasting for 3 days (P < 0.01). These results support the possibility that LLU-α is produced from γ- tocopherol in diet via oxidative metabolism without racemization. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)209-215
Number of pages7
JournalAnalytical Biochemistry
Issue number2
Publication statusPublished - 2000 Jun 1
Externally publishedYes



  • γ-CEHC
  • Chiral column
  • Coupled-column HPLC
  • DBD-PZ
  • Fluorometric detection
  • LLU-α

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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