Occurrence of β-endorphin binding sites in the pituitary of the frog Rana esculenta: Effect of β-endorphin on luteinizing hormone secretion

M. G. Sabbieti, L. Marchetti, G. Menghi, K. Yamamoto, S. Kikuyama, H. Vaudry, A. Polzonetti-Magni

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    The possible effect of proopiomelanocortin-derived peptide, β-endorphin on frog gonadotrope cells was investigated. Binding and internalization of β-endorphin to pituitary pars distalis cultured cells were visualized by immunofluorescence and analyzed by means of confocal laser scanning microscopy. Using biotinylated endorphin, the time-course of β-binding showed that this opioid was internalized through receptor-mediated endocytosis, the mechanism in which actin and clathrin were involved; then, the lysosomal degradation program occurred at later stages. The β-endorphin binding was well antagonized by Naloxone, the opiate receptor antagonist, and up-regulated since more rapid response was obtained in the previously primed cells. The double immunostaining reaction for β-endorphin and LH β-subunit revealed that half the β-endorphin labeled cell population was positively immunostained for LH β-subunit, and β-endorphin was able to induce an increasing trend of LH secretion in cultured pars distalis cells. Therefore, it seems that β-endorphin acts directly on pituitary pars distalis and influences gonadotropin secretion through the interaction with its own receptor.

    Original languageEnglish
    Pages (from-to)391-398
    Number of pages8
    JournalGeneral and Comparative Endocrinology
    Volume132
    Issue number3
    DOIs
    Publication statusPublished - 2003 Jul 1

    Fingerprint

    Rana esculenta
    Endorphins
    endorphins
    hormone secretion
    luteinizing hormone
    Luteinizing Hormone
    Anura
    frogs
    binding sites
    Binding Sites
    Anterior Pituitary Gland
    receptors
    secretion
    cells
    pro-opiomelanocortin
    clathrin
    naloxone
    Pro-Opiomelanocortin
    Clathrin
    narcotics

    Keywords

    • β-Endorphin
    • Binding sites
    • Frog
    • LH secretion

    ASJC Scopus subject areas

    • Endocrinology

    Cite this

    Occurrence of β-endorphin binding sites in the pituitary of the frog Rana esculenta : Effect of β-endorphin on luteinizing hormone secretion. / Sabbieti, M. G.; Marchetti, L.; Menghi, G.; Yamamoto, K.; Kikuyama, S.; Vaudry, H.; Polzonetti-Magni, A.

    In: General and Comparative Endocrinology, Vol. 132, No. 3, 01.07.2003, p. 391-398.

    Research output: Contribution to journalArticle

    Sabbieti, M. G. ; Marchetti, L. ; Menghi, G. ; Yamamoto, K. ; Kikuyama, S. ; Vaudry, H. ; Polzonetti-Magni, A. / Occurrence of β-endorphin binding sites in the pituitary of the frog Rana esculenta : Effect of β-endorphin on luteinizing hormone secretion. In: General and Comparative Endocrinology. 2003 ; Vol. 132, No. 3. pp. 391-398.
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