OFF-switching property of quorum sensor LuxR via As(III)-induced insoluble form

Rina Ayuba, Daisuke Umeno, Shigeko Kawai-Noma*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Whole-cell sensors for arsenite detection have been developed exclusively based on the natural arsenite (As(III)) sensory protein ArsR for arsenic metabolism. This study reports that the quorum-sensing LuxR/Plux system from Vibrio fischeri, which is completely unrelated to arsenic metabolism, responds to As(III) in a dose-dependent manner. Due to as many as 9 cysteine residues, which has a high binding affinity with As(III), LuxR underwent As(III)-induced insoluble form, thereby reducing its effective cellular concentration. Accordingly, the expression level of green fluorescent protein under the control of Plux gradually decreased with increasing As(III) concentration in the medium. This is a novel As(III)-detection system that has never been proposed before, with a unique ON-to-OFF transfer function.

Original languageEnglish
Pages (from-to)335-339
Number of pages5
JournalJournal of Bioscience and Bioengineering
Volume133
Issue number4
DOIs
Publication statusPublished - 2022 Apr

Keywords

  • Arsenite (As(III))
  • Cysteine
  • Protein aggregation
  • Quorum sensing
  • Whole-cell sensor

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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