TY - JOUR
T1 - OFF-switching property of quorum sensor LuxR via As(III)-induced insoluble form
AU - Ayuba, Rina
AU - Umeno, Daisuke
AU - Kawai-Noma, Shigeko
N1 - Funding Information:
This work was supported by Grant-in-Aid for Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology [JSPS Kakenhi-Grants 16H06450 , 18H01791 , 21H01721 ]. S. K.-N. is supported by Chiba University IMO 2021 Support Program for Research and Chiba University Research Promotion Program.
Publisher Copyright:
© 2021 The Society for Biotechnology, Japan
PY - 2022/4
Y1 - 2022/4
N2 - Whole-cell sensors for arsenite detection have been developed exclusively based on the natural arsenite (As(III)) sensory protein ArsR for arsenic metabolism. This study reports that the quorum-sensing LuxR/Plux system from Vibrio fischeri, which is completely unrelated to arsenic metabolism, responds to As(III) in a dose-dependent manner. Due to as many as 9 cysteine residues, which has a high binding affinity with As(III), LuxR underwent As(III)-induced insoluble form, thereby reducing its effective cellular concentration. Accordingly, the expression level of green fluorescent protein under the control of Plux gradually decreased with increasing As(III) concentration in the medium. This is a novel As(III)-detection system that has never been proposed before, with a unique ON-to-OFF transfer function.
AB - Whole-cell sensors for arsenite detection have been developed exclusively based on the natural arsenite (As(III)) sensory protein ArsR for arsenic metabolism. This study reports that the quorum-sensing LuxR/Plux system from Vibrio fischeri, which is completely unrelated to arsenic metabolism, responds to As(III) in a dose-dependent manner. Due to as many as 9 cysteine residues, which has a high binding affinity with As(III), LuxR underwent As(III)-induced insoluble form, thereby reducing its effective cellular concentration. Accordingly, the expression level of green fluorescent protein under the control of Plux gradually decreased with increasing As(III) concentration in the medium. This is a novel As(III)-detection system that has never been proposed before, with a unique ON-to-OFF transfer function.
KW - Arsenite (As(III))
KW - Cysteine
KW - Protein aggregation
KW - Quorum sensing
KW - Whole-cell sensor
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U2 - 10.1016/j.jbiosc.2021.12.013
DO - 10.1016/j.jbiosc.2021.12.013
M3 - Article
C2 - 35120813
AN - SCOPUS:85123889131
SN - 1389-1723
VL - 133
SP - 335
EP - 339
JO - Journal of Bioscience and Bioengineering
JF - Journal of Bioscience and Bioengineering
IS - 4
ER -