Oleed, a medaka polycomb group gene, regulates ciliogenesis and left-right patterning

Daisuke Arai, Atsushi Hatano, Toru Higashinakagawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Left-right (LR) patterning is an essential part of the animal body plan. Primary cilia are known to play a pivotal role in this process. In humans, genetic disorders of ciliogenesis cause serious congenital diseases. A comprehensive mechanism that regulates ciliogenesis has not been proposed so far. Here, we show that EED, a core member of the Polycomb group (PcG) genes and a presumed player in many epigenetic processes, is required for ciliogenesis and subsequent LR patterning in the medaka fish, Oryzias latipes. Moderate knockdown of oleed, a medaka homolog of EED, preferentially caused situs inversus. In the affected embryo, the cilia in Kupffer's vesicle showed various defects in their structure, position and motility. Furthermore, we demonstrated that oleed maintains the expression of Noto, which, in mice, regulates ciliogenesis and LR patterning. This study provides the first evidence for the involvement of epigenetic plasticity in LR patterning through ciliogenesis.

Original languageEnglish
Pages (from-to)1359-1367
Number of pages9
JournalGenes to Cells
Volume14
Issue number12
DOIs
Publication statusPublished - 2009 Dec

Fingerprint

Oryzias
Cilia
Genetic Epigenesis
Genes
Situs Inversus
Inborn Genetic Diseases
Medical Genetics
Human Body
Epigenomics
Fishes
Embryonic Structures

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Oleed, a medaka polycomb group gene, regulates ciliogenesis and left-right patterning. / Arai, Daisuke; Hatano, Atsushi; Higashinakagawa, Toru.

In: Genes to Cells, Vol. 14, No. 12, 12.2009, p. 1359-1367.

Research output: Contribution to journalArticle

Arai, Daisuke ; Hatano, Atsushi ; Higashinakagawa, Toru. / Oleed, a medaka polycomb group gene, regulates ciliogenesis and left-right patterning. In: Genes to Cells. 2009 ; Vol. 14, No. 12. pp. 1359-1367.
@article{1e7c8bb470974542aa07fa0747dc625f,
title = "Oleed, a medaka polycomb group gene, regulates ciliogenesis and left-right patterning",
abstract = "Left-right (LR) patterning is an essential part of the animal body plan. Primary cilia are known to play a pivotal role in this process. In humans, genetic disorders of ciliogenesis cause serious congenital diseases. A comprehensive mechanism that regulates ciliogenesis has not been proposed so far. Here, we show that EED, a core member of the Polycomb group (PcG) genes and a presumed player in many epigenetic processes, is required for ciliogenesis and subsequent LR patterning in the medaka fish, Oryzias latipes. Moderate knockdown of oleed, a medaka homolog of EED, preferentially caused situs inversus. In the affected embryo, the cilia in Kupffer's vesicle showed various defects in their structure, position and motility. Furthermore, we demonstrated that oleed maintains the expression of Noto, which, in mice, regulates ciliogenesis and LR patterning. This study provides the first evidence for the involvement of epigenetic plasticity in LR patterning through ciliogenesis.",
author = "Daisuke Arai and Atsushi Hatano and Toru Higashinakagawa",
year = "2009",
month = "12",
doi = "10.1111/j.1365-2443.2009.01353.x",
language = "English",
volume = "14",
pages = "1359--1367",
journal = "Genes to Cells",
issn = "1356-9597",
publisher = "Wiley-Blackwell",
number = "12",

}

TY - JOUR

T1 - Oleed, a medaka polycomb group gene, regulates ciliogenesis and left-right patterning

AU - Arai, Daisuke

AU - Hatano, Atsushi

AU - Higashinakagawa, Toru

PY - 2009/12

Y1 - 2009/12

N2 - Left-right (LR) patterning is an essential part of the animal body plan. Primary cilia are known to play a pivotal role in this process. In humans, genetic disorders of ciliogenesis cause serious congenital diseases. A comprehensive mechanism that regulates ciliogenesis has not been proposed so far. Here, we show that EED, a core member of the Polycomb group (PcG) genes and a presumed player in many epigenetic processes, is required for ciliogenesis and subsequent LR patterning in the medaka fish, Oryzias latipes. Moderate knockdown of oleed, a medaka homolog of EED, preferentially caused situs inversus. In the affected embryo, the cilia in Kupffer's vesicle showed various defects in their structure, position and motility. Furthermore, we demonstrated that oleed maintains the expression of Noto, which, in mice, regulates ciliogenesis and LR patterning. This study provides the first evidence for the involvement of epigenetic plasticity in LR patterning through ciliogenesis.

AB - Left-right (LR) patterning is an essential part of the animal body plan. Primary cilia are known to play a pivotal role in this process. In humans, genetic disorders of ciliogenesis cause serious congenital diseases. A comprehensive mechanism that regulates ciliogenesis has not been proposed so far. Here, we show that EED, a core member of the Polycomb group (PcG) genes and a presumed player in many epigenetic processes, is required for ciliogenesis and subsequent LR patterning in the medaka fish, Oryzias latipes. Moderate knockdown of oleed, a medaka homolog of EED, preferentially caused situs inversus. In the affected embryo, the cilia in Kupffer's vesicle showed various defects in their structure, position and motility. Furthermore, we demonstrated that oleed maintains the expression of Noto, which, in mice, regulates ciliogenesis and LR patterning. This study provides the first evidence for the involvement of epigenetic plasticity in LR patterning through ciliogenesis.

UR - http://www.scopus.com/inward/record.url?scp=71649109674&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71649109674&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2443.2009.01353.x

DO - 10.1111/j.1365-2443.2009.01353.x

M3 - Article

C2 - 19919648

AN - SCOPUS:71649109674

VL - 14

SP - 1359

EP - 1367

JO - Genes to Cells

JF - Genes to Cells

SN - 1356-9597

IS - 12

ER -