Oligomycin induced the proteasomal degradation of cyclin D1 protein

Mai Kanai; Satoru Iba; Ryoko Okada; Etsu Tashiro; Masaya Imoto

doi:10.1038/ja.2009.47

Oligomycin induced the proteasomal degradation of cyclin D1 protein

Mai Kanai, Satoru Iba, Ryoko Okada, Etsu Tashiro, Masaya Imoto^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

We searched for compounds that affect the cyclin D1/retinoblastoma protein pathway from the in-house natural product library using a recombinant adenovirus with the Cre/loxP-regulated cyclin D1 overexpression system, and we found that oligomycin inhibited cell growth more effectively in cyclin D1-overexpressing SW480 cells than in control SW480 cells. We also found that oligomycin reduced the expression levels of cyclin D1 protein and that this reduction is, at least in part, mediated by Thr-286 phosphorylation-dependent proteasomal degradation.

Original language	English
Pages (from-to)	425-429
Number of pages	5
Journal	Journal of Antibiotics
Volume	62
Issue number	8
DOIs	https://doi.org/10.1038/ja.2009.47
Publication status	Published - 2009 Aug 1
Externally published	Yes

Keywords

Cell growth inhibition
Chemosensitivity
Cyclin D1
E2F-1
PRB

ASJC Scopus subject areas

Pharmacology
Drug Discovery

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10.1038/ja.2009.47

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abstract = "We searched for compounds that affect the cyclin D1/retinoblastoma protein pathway from the in-house natural product library using a recombinant adenovirus with the Cre/loxP-regulated cyclin D1 overexpression system, and we found that oligomycin inhibited cell growth more effectively in cyclin D1-overexpressing SW480 cells than in control SW480 cells. We also found that oligomycin reduced the expression levels of cyclin D1 protein and that this reduction is, at least in part, mediated by Thr-286 phosphorylation-dependent proteasomal degradation.",

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AU - Kanai, Mai

AU - Iba, Satoru

AU - Okada, Ryoko

AU - Tashiro, Etsu

AU - Imoto, Masaya

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Y1 - 2009/8/1

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AB - We searched for compounds that affect the cyclin D1/retinoblastoma protein pathway from the in-house natural product library using a recombinant adenovirus with the Cre/loxP-regulated cyclin D1 overexpression system, and we found that oligomycin inhibited cell growth more effectively in cyclin D1-overexpressing SW480 cells than in control SW480 cells. We also found that oligomycin reduced the expression levels of cyclin D1 protein and that this reduction is, at least in part, mediated by Thr-286 phosphorylation-dependent proteasomal degradation.

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KW - Chemosensitivity

KW - Cyclin D1

KW - E2F-1

KW - PRB

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Oligomycin induced the proteasomal degradation of cyclin D1 protein

Abstract

Keywords

ASJC Scopus subject areas

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