Oligomycin induced the proteasomal degradation of cyclin D1 protein

Mai Kanai, Satoru Iba, Ryoko Okada, Etsu Tashiro, Masaya Imoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We searched for compounds that affect the cyclin D1/retinoblastoma protein pathway from the in-house natural product library using a recombinant adenovirus with the Cre/loxP-regulated cyclin D1 overexpression system, and we found that oligomycin inhibited cell growth more effectively in cyclin D1-overexpressing SW480 cells than in control SW480 cells. We also found that oligomycin reduced the expression levels of cyclin D1 protein and that this reduction is, at least in part, mediated by Thr-286 phosphorylation-dependent proteasomal degradation.

Original languageEnglish
Pages (from-to)425-429
Number of pages5
JournalJournal of Antibiotics
Volume62
Issue number8
DOIs
Publication statusPublished - 2009 Aug
Externally publishedYes

Fingerprint

Oligomycins
Cyclin D1
Proteins
Retinoblastoma Protein
Biological Products
Adenoviridae
Phosphorylation
Growth

Keywords

  • Cell growth inhibition
  • Chemosensitivity
  • Cyclin D1
  • E2F-1
  • PRB

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Oligomycin induced the proteasomal degradation of cyclin D1 protein. / Kanai, Mai; Iba, Satoru; Okada, Ryoko; Tashiro, Etsu; Imoto, Masaya.

In: Journal of Antibiotics, Vol. 62, No. 8, 08.2009, p. 425-429.

Research output: Contribution to journalArticle

Kanai, Mai ; Iba, Satoru ; Okada, Ryoko ; Tashiro, Etsu ; Imoto, Masaya. / Oligomycin induced the proteasomal degradation of cyclin D1 protein. In: Journal of Antibiotics. 2009 ; Vol. 62, No. 8. pp. 425-429.
@article{71fa85464941400589cfef24204d8e91,
title = "Oligomycin induced the proteasomal degradation of cyclin D1 protein",
abstract = "We searched for compounds that affect the cyclin D1/retinoblastoma protein pathway from the in-house natural product library using a recombinant adenovirus with the Cre/loxP-regulated cyclin D1 overexpression system, and we found that oligomycin inhibited cell growth more effectively in cyclin D1-overexpressing SW480 cells than in control SW480 cells. We also found that oligomycin reduced the expression levels of cyclin D1 protein and that this reduction is, at least in part, mediated by Thr-286 phosphorylation-dependent proteasomal degradation.",
keywords = "Cell growth inhibition, Chemosensitivity, Cyclin D1, E2F-1, PRB",
author = "Mai Kanai and Satoru Iba and Ryoko Okada and Etsu Tashiro and Masaya Imoto",
year = "2009",
month = "8",
doi = "10.1038/ja.2009.47",
language = "English",
volume = "62",
pages = "425--429",
journal = "Journal of Antibiotics",
issn = "0021-8820",
publisher = "Japan Antibiotics Research Association",
number = "8",

}

TY - JOUR

T1 - Oligomycin induced the proteasomal degradation of cyclin D1 protein

AU - Kanai, Mai

AU - Iba, Satoru

AU - Okada, Ryoko

AU - Tashiro, Etsu

AU - Imoto, Masaya

PY - 2009/8

Y1 - 2009/8

N2 - We searched for compounds that affect the cyclin D1/retinoblastoma protein pathway from the in-house natural product library using a recombinant adenovirus with the Cre/loxP-regulated cyclin D1 overexpression system, and we found that oligomycin inhibited cell growth more effectively in cyclin D1-overexpressing SW480 cells than in control SW480 cells. We also found that oligomycin reduced the expression levels of cyclin D1 protein and that this reduction is, at least in part, mediated by Thr-286 phosphorylation-dependent proteasomal degradation.

AB - We searched for compounds that affect the cyclin D1/retinoblastoma protein pathway from the in-house natural product library using a recombinant adenovirus with the Cre/loxP-regulated cyclin D1 overexpression system, and we found that oligomycin inhibited cell growth more effectively in cyclin D1-overexpressing SW480 cells than in control SW480 cells. We also found that oligomycin reduced the expression levels of cyclin D1 protein and that this reduction is, at least in part, mediated by Thr-286 phosphorylation-dependent proteasomal degradation.

KW - Cell growth inhibition

KW - Chemosensitivity

KW - Cyclin D1

KW - E2F-1

KW - PRB

UR - http://www.scopus.com/inward/record.url?scp=70249147253&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70249147253&partnerID=8YFLogxK

U2 - 10.1038/ja.2009.47

DO - 10.1038/ja.2009.47

M3 - Article

C2 - 19575044

AN - SCOPUS:70249147253

VL - 62

SP - 425

EP - 429

JO - Journal of Antibiotics

JF - Journal of Antibiotics

SN - 0021-8820

IS - 8

ER -