Oligonucleotide-arrayed TFT Photosensor Applicable for DNA chip technology

Tsuyoshi Tanaka, Keiichi Hatakeyama, Masahiro Sawaguchi, Akihito Iwadate, Yasushi Mizutani, Kazuhiro Sasaki, Naofumi Tateishi, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A thin film transistor (TFT) photosensor fabricated by semiconductor integrated circuit (IC) technology was applied to DNA chip technology. The surface of the TFT photosensor was coated with TiO2 using a vapor deposition technique for the fabrication of optical filters. The immobilization of thiolated oligonucleotide probes onto a Ti02-coated TFT photosensor using γ-aminopropyltriethoxysilane (APTES) and N-(γ-maleimidobutyloxy) sulfosuccinimide ester (GMBS) was optimized. The coverage value of immobilized oligonucleotides reached a plateau at 33.7 pmol/cm2, which was similar to a previous analysis using radioisotope-labeled oligonucleotides. The lowest detection limits were 0.05 pmol/cm2 for quantum dot and 2.1 pmol/cm2 for Alexa Fluor 350. Furthermore, single nucleotide polymorphism (SNP) detection was examined using the oligonucleotide-arrayed TFT photosensor. A SNP present in the aldehyde dehydrogenase 2 (ALDH2) gene was used as a target. The SNPs in ALDH2*1 and ALDH2*2 target DNA were detected successfully using the TFT photosensor. DNA hybridization in the presence of both ALDH2*1 and ALDH2*2 target DNA was observed using both ALDH2*1 and ALDH2*2 detection oligonucleotides-arrayed TFT photosensor. Use of the TFT photosensor will allow the development of a disposable photodetecting device for DNA chip systems.

Original languageEnglish
Pages (from-to)22-28
Number of pages7
JournalBiotechnology and bioengineering
Volume95
Issue number1
DOIs
Publication statusPublished - 2006 Sep 5

    Fingerprint

Keywords

  • Aldehyde dehydrogenase 2 (ALDH2) gene
  • DNA microarray
  • Single nucleotide polymorphism (SNP)
  • Thin film transistor (TFT) photosensor

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

Cite this

Tanaka, T., Hatakeyama, K., Sawaguchi, M., Iwadate, A., Mizutani, Y., Sasaki, K., Tateishi, N., Takeyama, H., & Matsunaga, T. (2006). Oligonucleotide-arrayed TFT Photosensor Applicable for DNA chip technology. Biotechnology and bioengineering, 95(1), 22-28. https://doi.org/10.1002/bit.20949