Oligonucleotide-arrayed TFT Photosensor Applicable for DNA chip technology

Tsuyoshi Tanaka, Keiichi Hatakeyama, Masahiro Sawaguchi, Akihito Iwadate, Yasushi Mizutani, Kazuhiro Sasaki, Naofumi Tateishi, Haruko Takeyama, Tadashi Matsunaga

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A thin film transistor (TFT) photosensor fabricated by semiconductor integrated circuit (IC) technology was applied to DNA chip technology. The surface of the TFT photosensor was coated with TiO2 using a vapor deposition technique for the fabrication of optical filters. The immobilization of thiolated oligonucleotide probes onto a Ti02-coated TFT photosensor using γ-aminopropyltriethoxysilane (APTES) and N-(γ-maleimidobutyloxy) sulfosuccinimide ester (GMBS) was optimized. The coverage value of immobilized oligonucleotides reached a plateau at 33.7 pmol/cm2, which was similar to a previous analysis using radioisotope-labeled oligonucleotides. The lowest detection limits were 0.05 pmol/cm2 for quantum dot and 2.1 pmol/cm2 for Alexa Fluor 350. Furthermore, single nucleotide polymorphism (SNP) detection was examined using the oligonucleotide-arrayed TFT photosensor. A SNP present in the aldehyde dehydrogenase 2 (ALDH2) gene was used as a target. The SNPs in ALDH2*1 and ALDH2*2 target DNA were detected successfully using the TFT photosensor. DNA hybridization in the presence of both ALDH2*1 and ALDH2*2 target DNA was observed using both ALDH2*1 and ALDH2*2 detection oligonucleotides-arrayed TFT photosensor. Use of the TFT photosensor will allow the development of a disposable photodetecting device for DNA chip systems.

Original languageEnglish
Pages (from-to)22-28
Number of pages7
JournalBiotechnology and Bioengineering
Volume95
Issue number1
DOIs
Publication statusPublished - 2006 Sep 5
Externally publishedYes

Fingerprint

Aldehyde Dehydrogenase
Oligonucleotides
Thin film transistors
Oligonucleotide Array Sequence Analysis
Aldehydes
DNA
Technology
Single Nucleotide Polymorphism
Immobilized Nucleic Acids
Quantum Dots
Semiconductors
Nucleotides
Oligonucleotide Probes
Polymorphism
Radioisotopes
Immobilization
Limit of Detection
Esters
Vapor deposition
Optical filters

Keywords

  • Aldehyde dehydrogenase 2 (ALDH2) gene
  • DNA microarray
  • Single nucleotide polymorphism (SNP)
  • Thin film transistor (TFT) photosensor

ASJC Scopus subject areas

  • Biotechnology
  • Microbiology

Cite this

Tanaka, T., Hatakeyama, K., Sawaguchi, M., Iwadate, A., Mizutani, Y., Sasaki, K., ... Matsunaga, T. (2006). Oligonucleotide-arrayed TFT Photosensor Applicable for DNA chip technology. Biotechnology and Bioengineering, 95(1), 22-28. https://doi.org/10.1002/bit.20949

Oligonucleotide-arrayed TFT Photosensor Applicable for DNA chip technology. / Tanaka, Tsuyoshi; Hatakeyama, Keiichi; Sawaguchi, Masahiro; Iwadate, Akihito; Mizutani, Yasushi; Sasaki, Kazuhiro; Tateishi, Naofumi; Takeyama, Haruko; Matsunaga, Tadashi.

In: Biotechnology and Bioengineering, Vol. 95, No. 1, 05.09.2006, p. 22-28.

Research output: Contribution to journalArticle

Tanaka, T, Hatakeyama, K, Sawaguchi, M, Iwadate, A, Mizutani, Y, Sasaki, K, Tateishi, N, Takeyama, H & Matsunaga, T 2006, 'Oligonucleotide-arrayed TFT Photosensor Applicable for DNA chip technology', Biotechnology and Bioengineering, vol. 95, no. 1, pp. 22-28. https://doi.org/10.1002/bit.20949
Tanaka T, Hatakeyama K, Sawaguchi M, Iwadate A, Mizutani Y, Sasaki K et al. Oligonucleotide-arrayed TFT Photosensor Applicable for DNA chip technology. Biotechnology and Bioengineering. 2006 Sep 5;95(1):22-28. https://doi.org/10.1002/bit.20949
Tanaka, Tsuyoshi ; Hatakeyama, Keiichi ; Sawaguchi, Masahiro ; Iwadate, Akihito ; Mizutani, Yasushi ; Sasaki, Kazuhiro ; Tateishi, Naofumi ; Takeyama, Haruko ; Matsunaga, Tadashi. / Oligonucleotide-arrayed TFT Photosensor Applicable for DNA chip technology. In: Biotechnology and Bioengineering. 2006 ; Vol. 95, No. 1. pp. 22-28.
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