On-chip cell sorting system using laser-induced heating of a thermoreversible gelation polymer to control flow

Yoshitaka Shirasaki, Jyunichi Tanaka, Hiroshi Makazu, Koichiro Tashiro, Shuichi Shoji, Shoichiro Tsukita, Takashi Funatsu

    Research output: Contribution to journalArticle

    78 Citations (Scopus)

    Abstract

    We have developed a microfabricated fluorescence-activated cell sorter system using a thermoreversible gelation polymer (TGP) as a switching valve. The glass sorter chip has Y-shaped microchannels with one inlet and two outlets. A biological specimen containing fluorescently labeled cells is mixed with a solution containing a thermoreversible sol-gel polymer. The mixed solution is then introduced into the sorter chip through the inlet. The sol-gel transformation was locally induced by site-directed infrared laser irradiation to plug one of the outlets. The fluorescently labeled target cells were detected with sensitive fluorescence microscopy. In the absence of a fluorescence signal, the collection channel is plugged through laser irradiation of the TGP and the specimens are directed to the waste channel. Upon detection of a fluorescence signal from the target cells, the laser beam is then used to plug the waste channel, allowing the fluorescent cells to be channeled into the collection reservoir. The response time of the sol-gel transformation was 3 ms, and a flow switching time of 120 ms was achieved. Using this system, we have demonstrated the sorting of fluorescent microspheres and Escherichia coli cells expressing fluorescent proteins. These cells were found to be viable after extraction from the sorting system, indicating no damage to the cells.

    Original languageEnglish
    Pages (from-to)695-701
    Number of pages7
    JournalAnalytical Chemistry
    Volume78
    Issue number3
    DOIs
    Publication statusPublished - 2006 Feb 1

    Fingerprint

    Gelation
    Sorting
    Flow control
    Sol-gels
    Polymers
    Fluorescence
    Cells
    Laser beam effects
    Heating
    Lasers
    Infrared lasers
    Fluorescence microscopy
    Microchannels
    Microspheres
    Escherichia coli
    Laser beams
    Glass
    Proteins

    ASJC Scopus subject areas

    • Analytical Chemistry

    Cite this

    On-chip cell sorting system using laser-induced heating of a thermoreversible gelation polymer to control flow. / Shirasaki, Yoshitaka; Tanaka, Jyunichi; Makazu, Hiroshi; Tashiro, Koichiro; Shoji, Shuichi; Tsukita, Shoichiro; Funatsu, Takashi.

    In: Analytical Chemistry, Vol. 78, No. 3, 01.02.2006, p. 695-701.

    Research output: Contribution to journalArticle

    Shirasaki, Yoshitaka ; Tanaka, Jyunichi ; Makazu, Hiroshi ; Tashiro, Koichiro ; Shoji, Shuichi ; Tsukita, Shoichiro ; Funatsu, Takashi. / On-chip cell sorting system using laser-induced heating of a thermoreversible gelation polymer to control flow. In: Analytical Chemistry. 2006 ; Vol. 78, No. 3. pp. 695-701.
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