On-chip cell sorting system using thermoreversible gelation polymer

Yoshitaka Shirasaki, Hirokazu Sugino, Masayasu Tatsuoka, Jun Mizuno, Shuichi Shoji, Takashi Funatsu

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We have developed a microfabricated fluorescence-activated cell sorter system using laminar flow of thermoreversible gelation polymer (TGP). The glass sorter chip consists of microchannels with two inlets for sample and buffer solutions, and two outlets for collection and waste of the specimen. A biological specimen containing fluorescently labeled cells, is mixed with a solution containing a TGP. The laminar flow of the mixed solution and buffer solution are then introduced into the sorter chip. The fluorescently labeled target cells were detected with sensitive fluorescence microscopy. In the absence of a fluorescence signal, the laminar flow of the specimen is directed into the waste channel. Upon detection of a fluorescence signal from the target cells, the sol-gel transformation was locally induced by site-directed infrared laser irradiation for the flow switching and for allowing the fluorescent cells to be channeled into the collection reservoir. The flow switching time of 100 ms was achieved. Using this system, we have demonstrated the sorting of Escherichia call cells expressing fluorescent proteins. These cells were found to be viable after extraction from the sorting system, indicating no damage to the cells.

Original languageEnglish
Pages (from-to)223-227
Number of pages5
JournalIEEE Journal on Selected Topics in Quantum Electronics
Volume13
Issue number2
DOIs
Publication statusPublished - 2007 Mar

Fingerprint

gelation
Gelation
classifying
Sorting
chips
Laminar flow
Cells
Fluorescence
polymers
Polymers
cells
laminar flow
fluorescence
Infrared lasers
Fluorescence microscopy
Laser beam effects
Microchannels
Sol-gels
buffers
Proteins

Keywords

  • Cell sorter
  • Flow cytometry
  • Microsystem

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics

Cite this

On-chip cell sorting system using thermoreversible gelation polymer. / Shirasaki, Yoshitaka; Sugino, Hirokazu; Tatsuoka, Masayasu; Mizuno, Jun; Shoji, Shuichi; Funatsu, Takashi.

In: IEEE Journal on Selected Topics in Quantum Electronics, Vol. 13, No. 2, 03.2007, p. 223-227.

Research output: Contribution to journalArticle

Shirasaki, Yoshitaka ; Sugino, Hirokazu ; Tatsuoka, Masayasu ; Mizuno, Jun ; Shoji, Shuichi ; Funatsu, Takashi. / On-chip cell sorting system using thermoreversible gelation polymer. In: IEEE Journal on Selected Topics in Quantum Electronics. 2007 ; Vol. 13, No. 2. pp. 223-227.
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