On-chip integration of solid-phase-extraction and silicon pillar arrays for high efficient liquid chromatography

K. Nakanishi, K. Shih, T. Kanamori, Donghyun Yoon, T. Funatsu, M. Tsunoda, Tetsushi Sekiguchi, Shuichi Shoji

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Silicon-based solid-phase-extraction (SPE) integrated liquid chromatography (LC) was proposed in this research. The integrated chip allowed continuous operation of selective sample enrichment and chromatography by a simple flow switching process. The sample reservoir was filled with extraction beads after silicon chip fabrication, and then targets were selectively adsorbed onto the beads. Pillar arrays provided uniform and dense sample band, while the targets were released from the beads. The integrated chip achieved more than 160 times higher fluorescence intensity of samples compared to normal injection chips, and coumarin 525 and 545 were successfully separated in 30-mm-long LC channel within 1 min.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1936-1938
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: 2015 Oct 252015 Oct 29

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period15/10/2515/10/29

Keywords

  • Liquid chromatography
  • On-chip integration
  • Silicon
  • Solid phase extraction

ASJC Scopus subject areas

  • Control and Systems Engineering

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