On-chip microcultivation chamber for swimming cells using visualized poly(dimethylsiloxane) valves

Kazunori Takahashi, Kazuki Orita, Kazunori Matsumura, Kenji Yasuda

Research output: Contribution to journalLetter

15 Citations (Scopus)

Abstract

We have developed a new type of on-chip microcultivation chamber made of poly(dimethylsiloxane) (PDMS) for long-term cultivation of swimming cells. The advantages of this chamber are that (1) the microfluidic channel width of the valve can be controlled according to air pressure while monitoring the microscopic image of the channel width, and that (2) a simple single-step procedure is required for the fabrication of the valve structure and microfluidic pathway. Using this chamber, we can control the passage of swimming Chlamydomonas cells, through the channel while visualizing the opening and closing of the valve as the medium buffer passes any time. Thus the long-term observation of the behavior of a particular single cell is accomplished.

Original languageEnglish
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume42
Issue number9 AB
Publication statusPublished - 2003 Sep 15
Externally publishedYes

Fingerprint

Polydimethylsiloxane
Microfluidics
chambers
chips
cells
closing
Fabrication
Monitoring
buffers
Air
fabrication
air
Swimming

Keywords

  • Air pressure
  • Chlamydomonas
  • Microcultivation chamber
  • Microscopic monitoring
  • Poly(dimethylsiloxane)
  • Swimming cells

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

On-chip microcultivation chamber for swimming cells using visualized poly(dimethylsiloxane) valves. / Takahashi, Kazunori; Orita, Kazuki; Matsumura, Kazunori; Yasuda, Kenji.

In: Japanese Journal of Applied Physics, Part 2: Letters, Vol. 42, No. 9 AB, 15.09.2003.

Research output: Contribution to journalLetter

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AU - Yasuda, Kenji

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N2 - We have developed a new type of on-chip microcultivation chamber made of poly(dimethylsiloxane) (PDMS) for long-term cultivation of swimming cells. The advantages of this chamber are that (1) the microfluidic channel width of the valve can be controlled according to air pressure while monitoring the microscopic image of the channel width, and that (2) a simple single-step procedure is required for the fabrication of the valve structure and microfluidic pathway. Using this chamber, we can control the passage of swimming Chlamydomonas cells, through the channel while visualizing the opening and closing of the valve as the medium buffer passes any time. Thus the long-term observation of the behavior of a particular single cell is accomplished.

AB - We have developed a new type of on-chip microcultivation chamber made of poly(dimethylsiloxane) (PDMS) for long-term cultivation of swimming cells. The advantages of this chamber are that (1) the microfluidic channel width of the valve can be controlled according to air pressure while monitoring the microscopic image of the channel width, and that (2) a simple single-step procedure is required for the fabrication of the valve structure and microfluidic pathway. Using this chamber, we can control the passage of swimming Chlamydomonas cells, through the channel while visualizing the opening and closing of the valve as the medium buffer passes any time. Thus the long-term observation of the behavior of a particular single cell is accomplished.

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KW - Microscopic monitoring

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