Abstract
The microfluidic platform is an important tool for diagnosis and biomedical studies because it enables us to handle precious cells and infectious materials safely. We have developed an on-chip microfluidic sorter with fluorescence spectrum detection and multiway separation. The fluorescence spectrum of specimens (495-685 nm) in the microchannels was obtained every 2 ms using a 1 × 16 arrayed photomultiplier tube. The specimen was identified by its spectrum and collected into the corresponding channel based on our previously reported thermoreversible gelation polymer technique (Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita and T. Funatsu, Anal. Chem., 2006, 78, 695-701). Four kinds of fluorescence microspheres and three kinds of Escherichia coli cells, expressing different fluorescent proteins, were successfully separated with accuracy and purity better than 90% at a throughput of about one particle per second.
| Original language | English |
|---|---|
| Pages (from-to) | 1254-1260 |
| Number of pages | 7 |
| Journal | Lab on a Chip - Miniaturisation for Chemistry and Biology |
| Volume | 9 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2009 |
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ASJC Scopus subject areas
- Biochemistry
- Chemistry(all)
- Bioengineering
- Biomedical Engineering
Cite this
On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation. / Sugino, Hirokazu; Ozaki, Kazuto; Shirasaki, Yoshitaka; Arakawa, Takahiro; Shoji, Shuichi; Funatsu, Takashi.
In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 9, No. 9, 2009, p. 1254-1260.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation
AU - Sugino, Hirokazu
AU - Ozaki, Kazuto
AU - Shirasaki, Yoshitaka
AU - Arakawa, Takahiro
AU - Shoji, Shuichi
AU - Funatsu, Takashi
PY - 2009
Y1 - 2009
N2 - The microfluidic platform is an important tool for diagnosis and biomedical studies because it enables us to handle precious cells and infectious materials safely. We have developed an on-chip microfluidic sorter with fluorescence spectrum detection and multiway separation. The fluorescence spectrum of specimens (495-685 nm) in the microchannels was obtained every 2 ms using a 1 × 16 arrayed photomultiplier tube. The specimen was identified by its spectrum and collected into the corresponding channel based on our previously reported thermoreversible gelation polymer technique (Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita and T. Funatsu, Anal. Chem., 2006, 78, 695-701). Four kinds of fluorescence microspheres and three kinds of Escherichia coli cells, expressing different fluorescent proteins, were successfully separated with accuracy and purity better than 90% at a throughput of about one particle per second.
AB - The microfluidic platform is an important tool for diagnosis and biomedical studies because it enables us to handle precious cells and infectious materials safely. We have developed an on-chip microfluidic sorter with fluorescence spectrum detection and multiway separation. The fluorescence spectrum of specimens (495-685 nm) in the microchannels was obtained every 2 ms using a 1 × 16 arrayed photomultiplier tube. The specimen was identified by its spectrum and collected into the corresponding channel based on our previously reported thermoreversible gelation polymer technique (Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita and T. Funatsu, Anal. Chem., 2006, 78, 695-701). Four kinds of fluorescence microspheres and three kinds of Escherichia coli cells, expressing different fluorescent proteins, were successfully separated with accuracy and purity better than 90% at a throughput of about one particle per second.
UR - http://www.scopus.com/inward/record.url?scp=65349115946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65349115946&partnerID=8YFLogxK
U2 - 10.1039/b815765k
DO - 10.1039/b815765k
M3 - Article
C2 - 19370245
AN - SCOPUS:65349115946
VL - 9
SP - 1254
EP - 1260
JO - Lab on a Chip - Miniaturisation for Chemistry and Biology
JF - Lab on a Chip - Miniaturisation for Chemistry and Biology
SN - 1473-0197
IS - 9
ER -