On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation

Hirokazu Sugino, Kazuto Ozaki, Yoshitaka Shirasaki, Takahiro Arakawa, Shuichi Shoji, Takashi Funatsu

    Research output: Contribution to journalArticle

    36 Citations (Scopus)

    Abstract

    The microfluidic platform is an important tool for diagnosis and biomedical studies because it enables us to handle precious cells and infectious materials safely. We have developed an on-chip microfluidic sorter with fluorescence spectrum detection and multiway separation. The fluorescence spectrum of specimens (495-685 nm) in the microchannels was obtained every 2 ms using a 1 × 16 arrayed photomultiplier tube. The specimen was identified by its spectrum and collected into the corresponding channel based on our previously reported thermoreversible gelation polymer technique (Y. Shirasaki, J. Tanaka, H. Makazu, K. Tashiro, S. Shoji, S. Tsukita and T. Funatsu, Anal. Chem., 2006, 78, 695-701). Four kinds of fluorescence microspheres and three kinds of Escherichia coli cells, expressing different fluorescent proteins, were successfully separated with accuracy and purity better than 90% at a throughput of about one particle per second.

    Original languageEnglish
    Pages (from-to)1254-1260
    Number of pages7
    JournalLab on a Chip - Miniaturisation for Chemistry and Biology
    Volume9
    Issue number9
    DOIs
    Publication statusPublished - 2009

    Fingerprint

    Microfluidics
    Sorting
    Fluorescence
    Photomultipliers
    Gelation
    Microchannels
    Microspheres
    Escherichia coli
    Polymers
    Throughput
    Proteins

    ASJC Scopus subject areas

    • Biochemistry
    • Chemistry(all)
    • Bioengineering
    • Biomedical Engineering

    Cite this

    On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation. / Sugino, Hirokazu; Ozaki, Kazuto; Shirasaki, Yoshitaka; Arakawa, Takahiro; Shoji, Shuichi; Funatsu, Takashi.

    In: Lab on a Chip - Miniaturisation for Chemistry and Biology, Vol. 9, No. 9, 2009, p. 1254-1260.

    Research output: Contribution to journalArticle

    Sugino, Hirokazu ; Ozaki, Kazuto ; Shirasaki, Yoshitaka ; Arakawa, Takahiro ; Shoji, Shuichi ; Funatsu, Takashi. / On-chip microfluidic sorting with fluorescence spectrum detection and multiway separation. In: Lab on a Chip - Miniaturisation for Chemistry and Biology. 2009 ; Vol. 9, No. 9. pp. 1254-1260.
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