Abstract
We have developed a novel method for on-chip cultivation of neural cells in a flexible agarose-microchamber array on a glass slide. The agarose microchamber is a micrometer-order cavity constructed on the surface of an agarose layer by molding a 50-μm-high square/circular micro-cast of thick SU-8 photoresist. In addition, the shape of the agarose microchamber was rearranged by using the photothermal etching method, in which we used an infrared (1064-nm) focused laser beam as the heat source to melt and remove a portion of agarose gel at the heating spot. With the photothermal etching method, we can also manufacture narrow tunnel-shaped channels between microchambers. When nerve cells were cultured on the agar-microchamber array chip, the nerve cells in two adjacent microchambers connected through the photothermal-etched channel after 48 hours of cultivation. Those results suggest the potential of an agarose-microchamber array integrated with the photothermal etching method for the next stage of single cell cultivation and measurement of nerve cells, such as real-time control of cell interactions during cultivation.
| Original language | English |
|---|---|
| Pages (from-to) | 37-42 |
| Number of pages | 6 |
| Journal | Electrical Engineering in Japan (English translation of Denki Gakkai Ronbunshi) |
| Volume | 146 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2004 Jan 30 |
| Externally published | Yes |
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Keywords
- Agarose
- Microchamber array
- Nerve cell
- Photothermal etching
- Shape rearrangement during cultivation
ASJC Scopus subject areas
- Electrical and Electronic Engineering
Cite this
On-chip neural cell cultivation using agarose-microchamber array constructed by a photothermal etching method. / Moriguchi, Hiroyuki; Takahashi, Kazunori; Sugio, Yoshihiro; Wakamoto, Yuichi; Inoue, Ippei; Jimbo, Yasuhiko; Yasuda, Kenji.
In: Electrical Engineering in Japan (English translation of Denki Gakkai Ronbunshi), Vol. 146, No. 2, 30.01.2004, p. 37-42.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - On-chip neural cell cultivation using agarose-microchamber array constructed by a photothermal etching method
AU - Moriguchi, Hiroyuki
AU - Takahashi, Kazunori
AU - Sugio, Yoshihiro
AU - Wakamoto, Yuichi
AU - Inoue, Ippei
AU - Jimbo, Yasuhiko
AU - Yasuda, Kenji
PY - 2004/1/30
Y1 - 2004/1/30
N2 - We have developed a novel method for on-chip cultivation of neural cells in a flexible agarose-microchamber array on a glass slide. The agarose microchamber is a micrometer-order cavity constructed on the surface of an agarose layer by molding a 50-μm-high square/circular micro-cast of thick SU-8 photoresist. In addition, the shape of the agarose microchamber was rearranged by using the photothermal etching method, in which we used an infrared (1064-nm) focused laser beam as the heat source to melt and remove a portion of agarose gel at the heating spot. With the photothermal etching method, we can also manufacture narrow tunnel-shaped channels between microchambers. When nerve cells were cultured on the agar-microchamber array chip, the nerve cells in two adjacent microchambers connected through the photothermal-etched channel after 48 hours of cultivation. Those results suggest the potential of an agarose-microchamber array integrated with the photothermal etching method for the next stage of single cell cultivation and measurement of nerve cells, such as real-time control of cell interactions during cultivation.
AB - We have developed a novel method for on-chip cultivation of neural cells in a flexible agarose-microchamber array on a glass slide. The agarose microchamber is a micrometer-order cavity constructed on the surface of an agarose layer by molding a 50-μm-high square/circular micro-cast of thick SU-8 photoresist. In addition, the shape of the agarose microchamber was rearranged by using the photothermal etching method, in which we used an infrared (1064-nm) focused laser beam as the heat source to melt and remove a portion of agarose gel at the heating spot. With the photothermal etching method, we can also manufacture narrow tunnel-shaped channels between microchambers. When nerve cells were cultured on the agar-microchamber array chip, the nerve cells in two adjacent microchambers connected through the photothermal-etched channel after 48 hours of cultivation. Those results suggest the potential of an agarose-microchamber array integrated with the photothermal etching method for the next stage of single cell cultivation and measurement of nerve cells, such as real-time control of cell interactions during cultivation.
KW - Agarose
KW - Microchamber array
KW - Nerve cell
KW - Photothermal etching
KW - Shape rearrangement during cultivation
UR - http://www.scopus.com/inward/record.url?scp=0346780346&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0346780346&partnerID=8YFLogxK
U2 - 10.1002/eej.10215
DO - 10.1002/eej.10215
M3 - Article
AN - SCOPUS:0346780346
VL - 146
SP - 37
EP - 42
JO - Electrical Engineering in Japan (English translation of Denki Gakkai Ronbunshi)
JF - Electrical Engineering in Japan (English translation of Denki Gakkai Ronbunshi)
SN - 0424-7760
IS - 2
ER -