On-chip pre-clinical cardiac toxicity

Testing compounds beyond herg and qt using hES/hiPS cardiomyocyte re-entry cell network model on a chip

Kenji Yasuda, T. Kaneko, F. Nomura

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Limitation of conventional hERG assay and QT prolongation testing for accurate prediction of Torsades de Pointes (TdP) by compounds unvailed us the necesity of new approach to evaluate global cardiac safety. On-chip re-entry cell network model assay has the potential to measure the TdP probability as pre-clinical testing for cardiac safety by the single cell-based optical/electrical measurement of the heart pressure, Na, K, Ca ion channel conditions and their fluctuations. The application of ion channel inhibitors resulted in dose-dependent changes to the field potential waveform, and these changes were identical to those induced in the native cardiomyocytes. This study shows that on-chip model represent a promising in vitro assay for cardiac toxicity and drug screening.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages716-718
Number of pages3
Volume2
Publication statusPublished - 2010
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen
Duration: 2010 Oct 32010 Oct 7

Other

Other14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CityGroningen
Period10/10/310/10/7

Fingerprint

Reentry
Toxicity
Assays
Testing
Ions
Screening

Keywords

  • Agarose microfabrication
  • Cardiac toxicity
  • Community effect
  • Field potential duration (FPD)
  • Functional reconstitution of cardiac cell network model
  • HES/hiPS delivered cardiomyocyte cells
  • Microelectrode array
  • On-chip cellomics
  • Poincaréplotting
  • QT prolongation
  • Re-entry model
  • Torsades de Pointes (TdP)

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Yasuda, K., Kaneko, T., & Nomura, F. (2010). On-chip pre-clinical cardiac toxicity: Testing compounds beyond herg and qt using hES/hiPS cardiomyocyte re-entry cell network model on a chip. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 2, pp. 716-718)

On-chip pre-clinical cardiac toxicity : Testing compounds beyond herg and qt using hES/hiPS cardiomyocyte re-entry cell network model on a chip. / Yasuda, Kenji; Kaneko, T.; Nomura, F.

14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2 2010. p. 716-718.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Yasuda, K, Kaneko, T & Nomura, F 2010, On-chip pre-clinical cardiac toxicity: Testing compounds beyond herg and qt using hES/hiPS cardiomyocyte re-entry cell network model on a chip. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 2, pp. 716-718, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, 10/10/3.
Yasuda K, Kaneko T, Nomura F. On-chip pre-clinical cardiac toxicity: Testing compounds beyond herg and qt using hES/hiPS cardiomyocyte re-entry cell network model on a chip. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2. 2010. p. 716-718
Yasuda, Kenji ; Kaneko, T. ; Nomura, F. / On-chip pre-clinical cardiac toxicity : Testing compounds beyond herg and qt using hES/hiPS cardiomyocyte re-entry cell network model on a chip. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2 2010. pp. 716-718
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