On-chip pre-clinical cardiac toxicity: Testing compounds beyond herg and qt using hES/hiPS cardiomyocyte re-entry cell network model on a chip

K. Yasuda, T. Kaneko, F. Nomura

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Limitation of conventional hERG assay and QT prolongation testing for accurate prediction of Torsades de Pointes (TdP) by compounds unvailed us the necesity of new approach to evaluate global cardiac safety. On-chip re-entry cell network model assay has the potential to measure the TdP probability as pre-clinical testing for cardiac safety by the single cell-based optical/electrical measurement of the heart pressure, Na, K, Ca ion channel conditions and their fluctuations. The application of ion channel inhibitors resulted in dose-dependent changes to the field potential waveform, and these changes were identical to those induced in the native cardiomyocytes. This study shows that on-chip model represent a promising in vitro assay for cardiac toxicity and drug screening.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages716-718
Number of pages3
Publication statusPublished - 2010 Dec 1
Externally publishedYes
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 2010 Oct 32010 Oct 7

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
CountryNetherlands
CityGroningen
Period10/10/310/10/7

Keywords

  • Agarose microfabrication
  • Cardiac toxicity
  • Community effect
  • Field potential duration (FPD)
  • Functional reconstitution of cardiac cell network model
  • HES/hiPS delivered cardiomyocyte cells
  • Microelectrode array
  • On-chip cellomics
  • Poincaréplotting
  • QT prolongation
  • Re-entry model
  • Torsades de Pointes (TdP)

ASJC Scopus subject areas

  • Control and Systems Engineering

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