On-chip single-cell-based microcultivation method for analysis of genetic information and epigenetic correlation of cells

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12 Citations (Scopus)

Abstract

We have developed an on-chip single-cell based microcultivation method for analyzing the variability of genetic information stored in single cells and their epigenetic correlations. The method uses four systems: an on-chip cell sorter for purifying the cells in a non-destructive manner; an on-chip single-cell cultivation chip for isolating single cells; an on-chip agarose microchamber system for constructive cell-cell network formation during cultivation; and an on-chip single-cell-based expression analysis system. Using these systems, we could measure the variability of prokaryotic cells and eukaryotic cells having the same DNA and found that, although prokaryotic cells have a large variability in their interdivision times, sister eukaryotic cells having the same DNA synchronized well. We also measured the dynamics of synchronization of beating cardiac myocytes and found that two isolated cells synchronize by one cell following the other after a short pause in beating. These results showed the potential of the on-chip microcultivation method's constructive approach to analyzing cell systems.

Original languageEnglish
Pages (from-to)186-193
Number of pages8
JournalJournal of Molecular Recognition
Volume17
Issue number3
DOIs
Publication statusPublished - 2004 May
Externally publishedYes

Fingerprint

Epigenomics
DNA
Sepharose
Synchronization
Prokaryotic Cells
Eukaryotic Cells
Cardiac Myocytes
System-on-chip

Keywords

  • Epigenetic correlation
  • Genetic information
  • On-chip agarose microchamber system
  • On-chip cell sorter
  • On-chip single-cell cultivation chip
  • On-chip single-cell-based expression analysis
  • On-chip single-cell-based microcultivation method

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Computer Vision and Pattern Recognition
  • Immunology
  • Molecular Biology

Cite this

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abstract = "We have developed an on-chip single-cell based microcultivation method for analyzing the variability of genetic information stored in single cells and their epigenetic correlations. The method uses four systems: an on-chip cell sorter for purifying the cells in a non-destructive manner; an on-chip single-cell cultivation chip for isolating single cells; an on-chip agarose microchamber system for constructive cell-cell network formation during cultivation; and an on-chip single-cell-based expression analysis system. Using these systems, we could measure the variability of prokaryotic cells and eukaryotic cells having the same DNA and found that, although prokaryotic cells have a large variability in their interdivision times, sister eukaryotic cells having the same DNA synchronized well. We also measured the dynamics of synchronization of beating cardiac myocytes and found that two isolated cells synchronize by one cell following the other after a short pause in beating. These results showed the potential of the on-chip microcultivation method's constructive approach to analyzing cell systems.",
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AB - We have developed an on-chip single-cell based microcultivation method for analyzing the variability of genetic information stored in single cells and their epigenetic correlations. The method uses four systems: an on-chip cell sorter for purifying the cells in a non-destructive manner; an on-chip single-cell cultivation chip for isolating single cells; an on-chip agarose microchamber system for constructive cell-cell network formation during cultivation; and an on-chip single-cell-based expression analysis system. Using these systems, we could measure the variability of prokaryotic cells and eukaryotic cells having the same DNA and found that, although prokaryotic cells have a large variability in their interdivision times, sister eukaryotic cells having the same DNA synchronized well. We also measured the dynamics of synchronization of beating cardiac myocytes and found that two isolated cells synchronize by one cell following the other after a short pause in beating. These results showed the potential of the on-chip microcultivation method's constructive approach to analyzing cell systems.

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KW - Genetic information

KW - On-chip agarose microchamber system

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KW - On-chip single-cell cultivation chip

KW - On-chip single-cell-based expression analysis

KW - On-chip single-cell-based microcultivation method

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