On-chip single-cell observation assay for propagation dynamics of yeast sup35 prionlike proteins

Satoru Ayano, Shigeko Noma, Masasuke Yoshida, Hideki Taguchi, Kenji Yasuda

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The cytoplasmically inherited genetic determinant [PSI+] of the yeast Sacchammyces cerevisiae is presumed to be a manifestation of the prionlike properties of the chromosome-encoded Sup35 protein. Here, we show the relationship between the cell growth and the inheritance of Sup35p-GFP aggregation using on-chip single-cell observation assay. When Sup35 was expressed by induction, an aggregation was started after soluble Sup35-GFP in the cytoplasm reached to the critical concentration. Once the aggregation was generated, the concentration of free Sup35-GFP remained constant at a critical value. After stopping the Sup35-GFP induction by changing the cultivation buffer, the concentration of soluble Sup35-GFP remained constant, whereas the size of aggregation decreased. This result indicates that the aggregation of Sup35-GFP is a reversible growth/shrinking phenomenon with the same velocity constant as that for a forward/reverse reaction.

Original languageEnglish
Pages (from-to)L1429-L1432
JournalJapanese Journal of Applied Physics, Part 2: Letters
Volume43
Issue number11 A
DOIs
Publication statusPublished - 2004 Nov 1

Keywords

  • Aggregation
  • Inheritance between generations
  • Microchamber
  • On-chip cultivation
  • Prion
  • Sacchammyces cerevisiae
  • Single cell
  • Sup35
  • Yeast

ASJC Scopus subject areas

  • Engineering(all)
  • Physics and Astronomy (miscellaneous)
  • Physics and Astronomy(all)

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