On the validity of the spectrophotometric determination of oxygen saturation of hemoglobin: The wavelength dependence of observed oxygen equilibrium parameter values

Kazuhiko Imaizumi, Kiyohiro Imai, Itiro Tyuma

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14 Citations (Scopus)

Abstract

Spectral changes of oxyhemoglobin induced by such anions as 2, 3-diphosphoglycerate, inositol hexaphosphate, and Cl- may affect the validity of the spectrophotometric determination of oxygen saturation of hemoglobin. Therefore, the anion-induced difference spectra were extensively measured under a variety of conditions and accurate oxygen equilibrium curves were determined under representative conditions with detection at different wavelengths selected from peaks, troughs, and zero difference points of the difference spectra in the visible and Soret regions. Oxygen equilibrium parameters including the four Adair constants (i.e., equilibrium constants for four steps of oxygenation) estimated from the equilibrium curves did not show any dependence on wavelength within the limits of experimental error. These results indicate that anion-induced spectral changes do not invalidate the spectrophotometric determination of oxygen saturation and confirm the validity of the previous conclusions drawn in our series of studies on the effects of anions, pH and temperature on oxygen equilibrium parameters.

Original languageEnglish
Pages (from-to)1707-1713
Number of pages7
JournalJournal of Biochemistry
Volume83
Issue number6
Publication statusPublished - 1978 Jun
Externally publishedYes

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ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

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