One-minute separation of biological compounds using pillar array column with low dispersion and low pressure-drop turns

Muneki Isokawa, Katsuya Takatsuki, Kailing Shih, Masanori Kono, Yanting Song, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a fast separation of biological compounds on pillar array columns with low dispersion and low pressure-drop (LD-LPD) turns using pressure-driven liquid chromatography. We found that a pillar array column with LD-LPD turns is useful for efficient and fast analysis of biological compounds. With high flow rate, one-minute separation of fluorescent derivatives of six amino acids was successfully performed. The analysis time was only one-fifth compared to a corresponding result using a pillar array column in a previous work. This result indicated that one-minute separation of complex mixtures could be realized using pillar array columns with LD-LPD turns.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages569-571
Number of pages3
Volume1
ISBN (Print)9781632666246
Publication statusPublished - 2013
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg
Duration: 2013 Oct 272013 Oct 31

Other

Other17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CityFreiburg
Period13/10/2713/10/31

Fingerprint

Pressure drop
Liquid chromatography
Complex Mixtures
Amino acids
Flow rate
Derivatives
Amino Acids

Keywords

  • Amino acids
  • Chip chromatography
  • Fluorescence
  • NBD-F
  • Pressure-driven

ASJC Scopus subject areas

  • Bioengineering

Cite this

Isokawa, M., Takatsuki, K., Shih, K., Kono, M., Song, Y., Sekiguchi, T., ... Tsunoda, M. (2013). One-minute separation of biological compounds using pillar array column with low dispersion and low pressure-drop turns. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (Vol. 1, pp. 569-571). Chemical and Biological Microsystems Society.

One-minute separation of biological compounds using pillar array column with low dispersion and low pressure-drop turns. / Isokawa, Muneki; Takatsuki, Katsuya; Shih, Kailing; Kono, Masanori; Song, Yanting; Sekiguchi, Tetsushi; Mizuno, Jun; Funatsu, Takashi; Shoji, Shuichi; Tsunoda, Makoto.

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. p. 569-571.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Isokawa, M, Takatsuki, K, Shih, K, Kono, M, Song, Y, Sekiguchi, T, Mizuno, J, Funatsu, T, Shoji, S & Tsunoda, M 2013, One-minute separation of biological compounds using pillar array column with low dispersion and low pressure-drop turns. in 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. vol. 1, Chemical and Biological Microsystems Society, pp. 569-571, 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013, Freiburg, 13/10/27.
Isokawa M, Takatsuki K, Shih K, Kono M, Song Y, Sekiguchi T et al. One-minute separation of biological compounds using pillar array column with low dispersion and low pressure-drop turns. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1. Chemical and Biological Microsystems Society. 2013. p. 569-571
Isokawa, Muneki ; Takatsuki, Katsuya ; Shih, Kailing ; Kono, Masanori ; Song, Yanting ; Sekiguchi, Tetsushi ; Mizuno, Jun ; Funatsu, Takashi ; Shoji, Shuichi ; Tsunoda, Makoto. / One-minute separation of biological compounds using pillar array column with low dispersion and low pressure-drop turns. 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013. Vol. 1 Chemical and Biological Microsystems Society, 2013. pp. 569-571
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