One-minute separation of biological compounds using pillar array column with low dispersion and low pressure-drop turns

Muneki Isokawa, Katsuya Takatsuki, Kailing Shih, Masanori Kono, Yanting Song, Tetsushi Sekiguchi, Jun Mizuno, Takashi Funatsu, Shuichi Shoji, Makoto Tsunoda

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a fast separation of biological compounds on pillar array columns with low dispersion and low pressure-drop (LD-LPD) turns using pressure-driven liquid chromatography. We found that a pillar array column with LD-LPD turns is useful for efficient and fast analysis of biological compounds. With high flow rate, one-minute separation of fluorescent derivatives of six amino acids was successfully performed. The analysis time was only one-fifth compared to a corresponding result using a pillar array column in a previous work. This result indicated that one-minute separation of complex mixtures could be realized using pillar array columns with LD-LPD turns.

Original languageEnglish
Title of host publication17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PublisherChemical and Biological Microsystems Society
Pages569-571
Number of pages3
ISBN (Print)9781632666246
Publication statusPublished - 2013 Jan 1
Event17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 - Freiburg, Germany
Duration: 2013 Oct 272013 Oct 31

Publication series

Name17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Volume1

Conference

Conference17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
CountryGermany
CityFreiburg
Period13/10/2713/10/31

Keywords

  • Amino acids
  • Chip chromatography
  • Fluorescence
  • NBD-F
  • Pressure-driven

ASJC Scopus subject areas

  • Bioengineering

Fingerprint Dive into the research topics of 'One-minute separation of biological compounds using pillar array column with low dispersion and low pressure-drop turns'. Together they form a unique fingerprint.

  • Cite this

    Isokawa, M., Takatsuki, K., Shih, K., Kono, M., Song, Y., Sekiguchi, T., Mizuno, J., Funatsu, T., Shoji, S., & Tsunoda, M. (2013). One-minute separation of biological compounds using pillar array column with low dispersion and low pressure-drop turns. In 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 (pp. 569-571). (17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013; Vol. 1). Chemical and Biological Microsystems Society.